CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis.

Pierre Calvel 1 Christine Kervarrec 1 Régis Lavigne 2 Virginie Vallet-Erdtmann 1 Myriam Guerrois 1 Antoine Rolland 1 Frédéric Chalmel 1 Bernard Jégou 1 Charles Pineau 1, 2, *
* Auteur correspondant
1 GERHM - Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes
2 Plate-forme protéomique [Rennes]
Abstract : In a recent proteomic study of rat spermatogenesis, we identified CLPH (for Casein-Like PHosphoprotein), a new testis-specific protein expressed exclusively in postmeiotic germ cells. In situ hybridization showed that the CLPH transcript was mainly present in round spermatids, whereas the protein was specifically detected by immunohistochemistry in elongated spermatids and in residual bodies. Electron microscopy showed the protein to be mostly cytoplasmic, but also frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation. The Clph gene was found to be present solely in mammalian genomes, in a chromosomal region syntenic to the mammalian cluster of secretory calcium-binding phosphoprotein (SCPP) genes. CLPH has several distinctive properties in common with SCPPs: calcium overlay experiments showed that CLPH was a calcium-binding protein, whereas trypsin digestion assay, circular dichroism and fluorescence experiments demonstrated its intrinsically disordered structure. We also showed that CLPH was phosphorylated in vitro and in vivo by casein kinase 2, an enzyme critical for spermatid elongation. Given the specific and strong production of CLPH during rat spermiogenesis, together with the particular biochemical properties of this protein, we suggest that CLPH is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.
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Article dans une revue
Journal of Proteome Research, American Chemical Society, 2009, 8 (6), pp.2953-65. 〈10.1021/pr900082m〉
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Contributeur : Christine Forgeron <>
Soumis le : jeudi 9 septembre 2010 - 11:50:30
Dernière modification le : mercredi 16 mai 2018 - 11:23:07

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IRSET | BIOSIT | UR1-UFR-SVE | IRSET-8 | IRSET-PROTIM | UNIV-RENNES

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Pierre Calvel, Christine Kervarrec, Régis Lavigne, Virginie Vallet-Erdtmann, Myriam Guerrois, et al.. CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis.. Journal of Proteome Research, American Chemical Society, 2009, 8 (6), pp.2953-65. 〈10.1021/pr900082m〉. 〈inserm-00516331〉

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