Skip to Main content Skip to Navigation
Journal articles

Development and implementation of standardized respiratory chain spectrophotometric assays for clinical diagnosis.

Abstract : Diversity of respiratory chain spectrophotometric assays may lead to difficult comparison of results between centers. The French network of mitochondrial diseases diagnostic centers undertook comparison of the results obtained with different protocols in the French diagnostic centers. The diversity of protocols was shown to have striking consequences, which prompted the network to undertake standardization and optimization of the protocols with respect to clinical diagnosis, i.e. high velocity while maintaining linear kinetics relative to time and enzyme concentration. Assays were set up on animal tissues and verified on control human muscle and fibroblasts. Influence of homogenization buffer and narrow range of optimal concentration of phosphate, substrate and tissue were shown. Experimental data and proposed protocols have been posted on a free access website. Their subsequent use in several diagnostic centers has improved consistency for all assays.
Document type :
Journal articles
Complete list of metadatas

Cited literature [29 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00516056
Contributor : Nathalie Roux-Buisson <>
Submitted on : Wednesday, September 8, 2010 - 4:08:10 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:04 AM
Long-term archiving on: : Friday, December 2, 2016 - 12:53:13 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Fadia Medja, S. Allouche, Paule Frachon, Claude Jardel, M. Malgat, et al.. Development and implementation of standardized respiratory chain spectrophotometric assays for clinical diagnosis.. Mitochondrion, Elsevier, 2009, 9 (5), pp.331-9. ⟨10.1016/j.mito.2009.05.001⟩. ⟨inserm-00516056⟩

Share

Metrics

Record views

302