Maintenance of red blood cell integrity by AMP-activated protein kinase alpha1 catalytic subunit.

Marc Foretz 1 Soizic Guihard 1 Jocelyne Leclerc 1 Véronique Fauveau 1 Jean-Pierre Couty 1 Fabienne Andris 2 Murielle Gaudry 1 Fabrizio Andreelli 1 Sophie Vaulont 1 Benoit Viollet 1, *
* Auteur correspondant
1 UM3 (UMR 8104 / U1016) - Institut Cochin
2 Laboratoire de Physiologie Animale
Abstract : AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy metabolism. We previously showed that AMPKalpha1-/- mice develop moderate anemia associated with splenomegaly and high reticulocytosis. Here, we report that splenectomy of AMPKalpha1-/- mice worsened anemia supporting evidence that AMPKalpha1-/- mice developed a compensatory response through extramedullary erythropoiesis in the spleen. Transplantation of bone marrow from AMPKalpha1-/- mice into wild-type recipients recapitulated the hematologic phenotype. Further, AMPKalpha1-/- red blood cells (RBC) showed less deformability in response to shear stress limiting their membrane flexibility. Thus, our results highlight the crucial role of AMPK to preserve RBC integrity.
Keywords : knockout mice Animal model AMPK Red blood cell Anemia Membrane Deformability
Type de document :
Article dans une revue
FEBS Letters, Wiley, 2010, 584 (16), pp.3667-71. 〈10.1016/j.febslet.2010.07.041〉
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Marc Foretz, Soizic Guihard, Jocelyne Leclerc, Véronique Fauveau, Jean-Pierre Couty, et al.. Maintenance of red blood cell integrity by AMP-activated protein kinase alpha1 catalytic subunit.. FEBS Letters, Wiley, 2010, 584 (16), pp.3667-71. 〈10.1016/j.febslet.2010.07.041〉. 〈inserm-00514852〉

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