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Journal articles
TCR stimulation drives cleavage and shedding of the ITIM receptor CD31.: CD31 cleavage and shedding from TCR-stimulated T-cells
Abstract : CD31 is a transmembrane molecule endowed with T cell regulatory functions owing to the presence of 2 immunotyrosine-based inhibitory motifs. For reasons not understood, CD31 is lost by a portion of circulating T lymphocytes, which appear prone to uncontrolled activation. In this study, we show that extracellular T cell CD31 comprising Ig-like domains 1 to 5 is cleaved and shed from the surface of human T cells upon activation via their TCR. The shed CD31 can be specifically detected as a soluble, truncated protein in human plasma. CD31 shedding results in the loss of its inhibitory function because the necessary cis-homo-oligomerization of the molecule, triggered by the trans-homophilic engagement of the distal Ig-like domain 1, cannot be established by CD31(shed) cells. However, we show that a juxta-membrane extracellular sequence, comprising part of the domain 6, remains expressed at the surface of CD31(shed) T cells. We also show that the immunosuppressive CD31 peptide aa 551-574 is highly homophilic and possibly acts by homo-oligomerizing with the truncated CD31 remaining after its cleavage and shedding. This peptide is able to sustain phosphorylation of the CD31 ITIM(686) and of SHP2 and to inhibit TCR-induced T cell activation. Finally, systemic administration of the peptide in BALB/c mice efficiently suppresses Ag-induced T cell-mediated immune responses in vivo. We conclude that the loss of T cell regulation caused by CD31 shedding driven by TCR stimulation can be rescued by molecular tools able to engage the truncated juxta-membrane extracellular molecule that remains exposed at the surface of CD31(shed) cells.
Cited literature [39 references]
https://www.hal.inserm.fr/inserm-00512366
Contributor : Giuseppina Caligiuri <>
Submitted on : Wednesday, April 20, 2011 - 8:41:33 AM
Last modification on : Wednesday, December 9, 2020 - 3:45:49 AM
Long-term archiving on: : Thursday, July 21, 2011 - 2:24:26 AM
Contributor : Giuseppina Caligiuri <>
Submitted on : Wednesday, April 20, 2011 - 8:41:33 AM
Last modification on : Wednesday, December 9, 2020 - 3:45:49 AM
Long-term archiving on: : Thursday, July 21, 2011 - 2:24:26 AM
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