Identification of specific protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family. - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2010

Identification of specific protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family.

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Abstract

In order to determine how gene expression is regulated in response to environmental cues, it is necessary to identify the specific interaction between transcription factors and their cognate cis-regulatory DNA elements. Here we have out-lined electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) protocols to define in vitro and in vivo USFs specific interacting sequences. The proposed procedures have been optimized for the USFs transcription factor family, allowing the identification of USF-specific targets.
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inserm-00511968 , version 1 (26-08-2010)

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Marie-Dominique Galibert, Yorann Baron. Identification of specific protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family.. Methods in Molecular Biology, 2010, 647, pp.391-406. ⟨10.1007/978-1-60761-738-9_24⟩. ⟨inserm-00511968⟩
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