Osteopontin expression in cardiomyocytes induces dilated cardiomyopathy. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Circulation. Heart failure Year : 2010

Osteopontin expression in cardiomyocytes induces dilated cardiomyopathy.


BACKGROUND: Inflammatory processes play a critical role in myocarditis, dilated cardiomyopathy, and heart failure. The expression of the inflammatory chemokine osteopontin (OPN) is dramatically increased in cardiomyocytes and inflammatory cells during myocarditis and heart failure in human and animals. However, its role in the development of heart diseases is not known. METHODS AND RESULTS: To understand whether OPN is involved in cardiomyopathies, we generated a transgenic mouse (MHC-OPN) that specifically overexpresses OPN in cardiomyocytes with cardiac-specific promoter-directed OPN expression. Young MHC-OPN mice were phenotypically indistinguishable from their control littermates, but most of them died prematurely with a half-life of 12 weeks of age. Electrocardiography revealed conduction defects. Echocardiography showed left ventricular dilation and systolic dysfunction. Histological analysis revealed cardiomyocyte loss, severe fibrosis, and inflammatory cell infiltration. Most of these inflammatory cells were activated T cells with Th1 polarization and cytotoxic activity. Autoantibodies against OPN, cardiac myosin, or troponin I, were not found in the serum of MHC-OPN mice. CONCLUSIONS: These data show that OPN expression in the heart induces in vivo T-cell recruitment and activation leading to chronic myocarditis, the consequence of which is myocyte destruction and hence, dilated cardiomyopathy. Thus, OPN might therefore constitute a potential therapeutic target to limit heart failure.

Dates and versions

inserm-00504854 , version 1 (21-07-2010)



Marie-Ange Renault, Fanny Robbesyn, Patricia Réant, Victorine Douin, Danièle Daret, et al.. Osteopontin expression in cardiomyocytes induces dilated cardiomyopathy.. Circulation. Heart failure, 2010, 3 (3), pp.431-9. ⟨10.1161/CIRCHEARTFAILURE.109.898114⟩. ⟨inserm-00504854⟩
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