Alix is involved in caspase 9 activation during calcium-induced apoptosis.

Flavie Strappazzon 1 Sakina Torch 1 Christine Chatellard-Causse 1 Anne Petiot 1 Chantal Thibert 1 Béatrice Blot 1 Jean-Marc Verna 1 Rémy Sadoul 1, *
* Auteur correspondant
1 GIN - Grenoble Institut des Neurosciences
Abstract : The cytoplasmic protein Alix/AIP1 (ALG-2 interacting protein X) is involved in cell death through mechanisms which remain unclear but require its binding partner ALG-2 (apoptosis-linked gene-2). The latter was defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. We show here that Alix is also a critical component of caspase 9 activation and apoptosis triggered by calcium. Indeed, expression of Alix dominant-negative mutants or downregulation of Alix afford significant protection against cytosolic calcium elevation following thapsigargin (Tg) treatment. The function of Alix in this paradigm requires its interaction with ALG-2. In addition, we demonstrate that caspase 9 activation is necessary for apoptosis induced by Tg and that this activation is impaired by knocking down Alix. Altogether, our findings identify, for the first time, Alix as a crucial mediator of Ca(2+) induced caspase 9 activation.
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Article dans une revue
Biochemical and Biophysical Research Communications, Elsevier, 2010, 397 (1), pp.64-9. 〈10.1016/j.bbrc.2010.05.062〉
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Flavie Strappazzon, Sakina Torch, Christine Chatellard-Causse, Anne Petiot, Chantal Thibert, et al.. Alix is involved in caspase 9 activation during calcium-induced apoptosis.. Biochemical and Biophysical Research Communications, Elsevier, 2010, 397 (1), pp.64-9. 〈10.1016/j.bbrc.2010.05.062〉. 〈inserm-00498756〉

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