Mapping 3-dimensional neovessel organization steps using micro-computed tomography in a murine model of hindlimb ischemia-brief report. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Arteriosclerosis, Thrombosis, and Vascular Biology Année : 2009

Mapping 3-dimensional neovessel organization steps using micro-computed tomography in a murine model of hindlimb ischemia-brief report.

Résumé

OBJECTIVE: Studying the mechanisms of neovascularization and evaluating the effects of proangiogenic strategies require accurate analysis of the neovascular network. We sought to evaluate the contribution of the microcomputed tomography (mCT) providing high-resolution 3-dimensional (3D) structural data, to a better comprehension of the well-studied mouse hindlimb postischemic neovascularization. METHODS AND RESULTS: We showed a predominant arteriogenesis process in the thigh and a predominant angiogenesis-related process in the tibiofibular region, in response to ischemia during the first 15 days. After 15 days, mCT quantitative analysis reveals a remodeling of arterial neovessels and a regression depending on the restoration of the blood flow. We provided also new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization. We discussed the contribution of this technique compared with or in addition to data generated by the more conventional approaches. CONCLUSIONS: This study demonstrated that optimized mCT is a robust method for providing new insights into the 3D understanding of postischemic vessel formation.
Fichier principal
Vignette du fichier
inserm-00497033_edited.pdf (231.61 Ko) Télécharger le fichier
Short_report_Couffinhal_250509_.pdf (28.68 Ko) Télécharger le fichier
Supplementary_data250509.doc (184 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Format : Autre

Dates et versions

inserm-00497033 , version 1 (02-07-2010)

Identifiants

Citer

Pierre Oses, Marie-Ange Renault, Rémi Chauvel, Lionel Leroux, Pierre Chevallereau, et al.. Mapping 3-dimensional neovessel organization steps using micro-computed tomography in a murine model of hindlimb ischemia-brief report.. Arteriosclerosis, Thrombosis, and Vascular Biology, 2009, 29 (12), pp.2090-2. ⟨10.1161/ATVBAHA.109.192732⟩. ⟨inserm-00497033⟩

Collections

INSERM FRM
183 Consultations
829 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More