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Raman spectroscopy: a novel experimental approach to evaluating renal tumours.

Abstract : BACKGROUND: New optical techniques of spectroscopy have shown promising results in the evaluation of solid tumours. OBJECTIVE: To evaluate the potential of Raman spectroscopy (RS) to assess renal tumours at surgery. DESIGN, SETTING, AND PARTICIPANTS: Over a 5-mo period, Raman optical spectra were prospectively acquired on surgical renal specimens removed due to suspicion of cancer. MEASUREMENTS: Raman measures were normalised to ensure comparison between spectra. A lower resolution signal was computed using a wavelet decomposition procedure to diminish the size of the signal and exploit the complete spectrum. A support vector machine (SVM) with a linear kernel and a sequential minimal optimisation solver was applied. A leave-one-out cross-validation technique was used to train and test the SVM. RESULTS AND LIMITATIONS: There were 36 patients with 34 malignant tumours (27 clear-cell, 6 papillary, and 1 chromophobe) and 2 benign (1 oncocytoma and 1 metanephric cyst) tumours. A total of 241 analysable Raman spectra were obtained. The SVM was able to classify tumoural and normal tissue with an accuracy of 84% (sensitivity 82%, specificity 87%). High-grade and low-grade tumours were differentiated with a precision of 82% (sensitivity 84%, specificity 80%). Histologic subtype could be categorised with an accuracy of 93% (sensitivity 96%, specificity 87%). SVM could not be applied to classify benign and malignant tumours because of the restricted number of benign spectra. CONCLUSIONS: RS can accurately differentiate normal and tumoural renal tissue, low-grade and high-grade renal tumours, and histologic subtype of renal cell carcinoma. Larger prospective studies are needed to confirm these preliminary data.
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Submitted on : Sunday, October 3, 2010 - 8:33:51 PM
Last modification on : Thursday, March 5, 2020 - 5:04:01 PM
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Karim Bensalah, Julien Fleureau, Denis Rolland, Olivier Lavastre, Nathalie Rioux-Leclercq, et al.. Raman spectroscopy: a novel experimental approach to evaluating renal tumours.. European Urology, Elsevier, 2010, 58 (4), pp.602-8. ⟨10.1016/j.eururo.2010.06.002⟩. ⟨inserm-00496624⟩

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