Skip to Main content Skip to Navigation
Journal articles

Three-independent-compartment chamber to study in vitro commissural synapses.

Abstract : We describe a novel chamber in which the two intact neonatal rat hippocampi and the commissural fibers are placed in three independent compartments separated by latex membranes and perfused selectively with different solutions. A set of control tests showed that the compartments are well isolated: 1) methylene blue or eosin applied to one compartment did not diffuse to other compartments when verified via the microscope, and spectrophotometry revealed that <1/10.000th of the dye diffuses to other compartments; 2) tetrodotoxin (1 microM) applied to the commissural compartment blocked the synaptic responses evoked contralaterally without affecting those evoked on the ipsilateral side. This chamber enables a wide range of experiments that cannot be performed in conventional chambers, e.g., to study the maturation and plasticity of the commissural connections, bilateral synchronization of the rhythmic activities in the limbic system, commissural propagation of the epileptiform activities, etc.
Document type :
Journal articles
Complete list of metadatas

Cited literature [4 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00487282
Contributor : Roman Tyzio <>
Submitted on : Friday, May 28, 2010 - 3:11:47 PM
Last modification on : Friday, March 27, 2020 - 2:23:29 AM
Long-term archiving on: : Thursday, March 30, 2017 - 12:08:29 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

  • HAL Id : inserm-00487282, version 1
  • PUBMED : 10036300

Collections

Citation

Roustem Khazipov, Luc Desfreres, Ilgam Khalilov, Yehezkel Ben-Ari. Three-independent-compartment chamber to study in vitro commissural synapses.. Journal of Neurophysiology, American Physiological Society, 1999, 81 (2), pp.921-4. ⟨inserm-00487282⟩

Share

Metrics

Record views

153