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Leukemia inhibitory factor: Role in human mesenchymal stem cells mediated immunosuppression.

Abstract : The interactions between mesenchymal stem cells (MSCs) and immune system are currently being explored. Leukemia inhibitory factor (LIF) is linked to regulatory transplantation tolerance. Our aim was to study the expression of LIF on human MSCs at both gene and protein level in mixed lymphocyte reaction (MSC/MLR), and its implication in MSC immunosuppressive effect. There was a 7-fold increase (611pg/ml) in LIF in MSC/MLR as compared to MSCs alone. Using LIF neutralizing antibody, a significant restoration of up to 91% of CD3+ lymphocyte proliferation in MSC/MLR was observed (p=0.021). LIF was implicated in the generation of regulatory lymphocytes, as demonstrated by decrease of Foxp3+ regulatory cells after using LIF neutralizing antibody in MSC/MLR (p=0.06) by flow cytometry. A positive correlation between LIF and human leukocyte antigen (HLA-G) gene expression by MSCs was found (R(2)=0.74). Our findings provide evidence supporting the immunomodulatory effect of MSCs.
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Contributor : Philippe Saas Connect in order to contact the contributor
Submitted on : Wednesday, May 19, 2010 - 12:47:54 PM
Last modification on : Friday, August 5, 2022 - 3:40:31 AM

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Aisha Nasef, Christelle Mazurier, Sandrine Bouchet, Sabine François, Alain Chapel, et al.. Leukemia inhibitory factor: Role in human mesenchymal stem cells mediated immunosuppression.. Cellular Immunology, Elsevier, 2008, 253 (1-2), pp.16-22. ⟨10.1016/j.cellimm.2008.06.002⟩. ⟨inserm-00484858⟩



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