Human cord blood-derived hematopoietic and neural-like stem/progenitor cells are attracted by the neurotransmitter GABA.

Vincent Zangiacomi 1 Norbert Balon 1 Stéphane Maddens 1 Pierre Tiberghien 2 Claudine Versaux-Botteri 1 Frédéric Deschaseaux 1
1 CRC (UMR_S 872) - Centre de Recherche des Cordeliers
2 HOTE GREFFON - Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098)
Abstract : Migration of stem/progenitor cells is a crucial event for homing toward tissue where cells need to be renewed. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to have a crucial role in migration of neuronal stem/progenitor cells. Since human umbilical cord blood (HUCB) contains stem/progenitor cells able to generate either neuronal or hematopoietic cells, we evaluated the effect of GABA on this type of cells. While whole fraction of mononuclear cells expressed GABA(A) and GABA(B) receptor subunits (GABA-R), only GABA(B)R subunits were found to be expressed on immature CD133+ cells. Functional experiments revealed that both cell fractions of HUCB were attracted by a gradient of GABA concentration and furthermore were blocked by specific antagonists of GABA(A)R and GABA(B)R bicuculline and saclofen, respectively. Moreover, through GABA(B)R activation the migrating fraction was highly enriched by both hematopoietic progenitors and cells able to generate neuron- like cells in culture. Therefore, GABA is a potent chemoattractant of HUCB stem/progenitor cells specifically through GABA(B)R activation.
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Article dans une revue
Stem Cells and Development, Mary Ann Liebert, 2009, 18 (9), pp.1369-78. 〈10.1089/scd.2008.0367〉
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http://www.hal.inserm.fr/inserm-00484491
Contributeur : Philippe Saas <>
Soumis le : mardi 18 mai 2010 - 15:00:28
Dernière modification le : lundi 9 juillet 2018 - 13:12:02

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UNIV-FCOMTE | UMR645 | UPMC | UNIV-PARIS5 | CORDELIERS | USPC

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Vincent Zangiacomi, Norbert Balon, Stéphane Maddens, Pierre Tiberghien, Claudine Versaux-Botteri, et al.. Human cord blood-derived hematopoietic and neural-like stem/progenitor cells are attracted by the neurotransmitter GABA.. Stem Cells and Development, Mary Ann Liebert, 2009, 18 (9), pp.1369-78. 〈10.1089/scd.2008.0367〉. 〈inserm-00484491〉

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