Skip to Main content Skip to Navigation
Journal articles

CD3-specific antibody-induced immune tolerance involves transforming growth factor-beta from phagocytes digesting apoptotic T cells.

Abstract : Intact CD3-specific antibody transiently depletes large numbers of T cells and subsequently induces long-term immune tolerance. The underlying mechanisms for the systemic tolerance, however, remain unclear. We show here that treatment of normal mice with intact antibody to CD3 increases systemic transforming growth factor-beta (TGF-beta) produced by phagocytes exposed to apoptotic T cells. Among the phagocytes, macrophages and immature dendritic cells (iDCs) secrete TGF-beta upon ingestion of apoptotic T cells, which induces CD4+Foxp3+ regulatory T cells in culture and contributes to immune tolerance mediated by CD3-specific antibody in vivo. In accordance with these results, depletion of macrophages and iDCs not only abrogates CD3-specific antibody-mediated prevention of myelin oligodendrocyte glycoprotein-induced acute experimental autoimmune encephalomyelitis (EAE), but also reverses the therapeutic effects of antibody to CD3 on established disease in a model of relapsing-remitting EAE. Thus, CD3-specific antibody-induced immune tolerance is associated with TGF-beta production in phagocytes involved in clearing apoptotic T cells, which suggests that apoptosis is linked to active suppression in immune tolerance.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00483517
Contributor : Philippe Saas <>
Submitted on : Friday, May 14, 2010 - 1:55:11 PM
Last modification on : Wednesday, September 16, 2020 - 10:43:29 AM

Identifiers

Collections

Citation

Sylvain Perruche, Pin Zhang, Yongzhong Liu, Philippe Saas, Jeffrey Bluestone, et al.. CD3-specific antibody-induced immune tolerance involves transforming growth factor-beta from phagocytes digesting apoptotic T cells.. Nature Medicine, Nature Publishing Group, 2008, 14 (5), pp.528-35. ⟨10.1038/nm1749⟩. ⟨inserm-00483517⟩

Share

Metrics

Record views

111