Skip to Main content Skip to Navigation
Journal articles

Bumetanide, an NKCC1 antagonist, does not prevent formation of epileptogenic focus but blocks epileptic focus seizures in immature rat hippocampus.

Abstract : Excitatory GABA action induced by high [Cl(-)](i) is thought to contribute to seizure generation in neonatal neurons although the mechanism of this effect remains unclear. We report that bumetanide, a NKCC1 antagonist, reduces driving force of GABA-mediated currents (DF(GABA)) in neonatal hippocampal neurons and blocks the giant depolarizing potentials (GDPs), a spontaneous pattern of network activity. In the preparation composed of two intact interconnected hippocampi, bumetanide did not prevent generation of kainate-induced seizures, their propagation to the contralateral hippocampus, and formation of an epileptogenic mirror focus. However, in the isolated mirror focus, bumetanide effectively blocked spontaneous epileptiform activity transforming it to the GDP-like activity pattern. Bumetanide partially reduced DF(GABA) and therefore the excitatory action of GABA in epileptic neurons. Therefore bumetanide is a potent anticonvulsive agent although it cannot prevent formation of the epileptogenic mirror focus. We suggest that an additional mechanism other than NKCC1-mediated contributes to the persistent increase of DF(GABA) in epileptic neurons.
Document type :
Journal articles
Complete list of metadatas

Cited literature [18 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00483209
Contributor : Roman Tyzio <>
Submitted on : Wednesday, May 12, 2010 - 5:52:26 PM
Last modification on : Tuesday, February 4, 2020 - 10:00:04 AM
Long-term archiving on: : Thursday, December 1, 2016 - 12:25:27 AM

File

Nardou_Khalilov_bumetanide_200...
Files produced by the author(s)

Identifiers

Collections

Citation

Romain Nardou, Yehezkel Ben-Ari, Ilgam Khalilov. Bumetanide, an NKCC1 antagonist, does not prevent formation of epileptogenic focus but blocks epileptic focus seizures in immature rat hippocampus.. Journal of Neurophysiology, American Physiological Society, 2009, 101 (6), pp.2878-88. ⟨10.1152/jn.90761.2008⟩. ⟨inserm-00483209⟩

Share

Metrics

Record views

180

Files downloads

301