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Journal articles
Increase of CD4+ CD25+ regulatory T cells in the peripheral blood of patients with metastatic carcinoma: a Phase I clinical trial using cyclophosphamide and immunotherapy to eliminate CD4+ CD25+ T lymphocytes.
S. Audia
1
A. Nicolas
1
D. Cathelin
1
N. Larmonier
2
Christophe Ferrand
3
P. Foucher
4
A. Fanton
4
E. Bergoin
4
M. Maynadie
4
L. Arnould
5
A. Bateman
6
B. Lorcerie
4
E. Solary
1
B. Chauffert
1
B. Bonnotte
1
1
LNC - Lipides - Nutrition - Cancer (U866) (Université de Bourgogne - Faculte de medecine - 7, boulevard Jeanne d'Arc - BP 87900 - 21079 Dijon Cedex - France)
- UB - Université de Bourgogne (Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U866 (101, rue de Tolbiac, 75013 Paris - France)
- AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement : U866 (26 Boulevard du Dr Petitjean - BP 87999 - 21079 Dijon cedex - France)
- ENSBANA - Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon : U866 (Campus de Montmuzard, 21100 Dijon - France)
2
Department of Paediatrics (Tucson, AZ, USA - United States)
- University of Arizona (Tucson AZ 85721 USA - United States)
3
HOTE GREFFON - Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (I.B.C.T.
Bâtiment Inserm
240 Route de Dole/Rue F. Dolto
25000 Besançon - France)
- UFC - Université de Franche-Comté (France)
- EFS [Bourgogne-France-Comté] - Etablissement français du sang [Bourgogne-France-Comté] (1 Boulevard Fleming 25020 Besançon cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1098 (101, rue de Tolbiac, 75013 Paris - France)
4
Hôpital du Bocage (2, boulevard Maréchal-de-Lattre-de-Tassigny, 21000 Dijon - France)
- CHU Dijon - Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon - 14 rue Paul Gaffarel - 21079 Dijon - France)
5
UNICANCER/CRLCC-CGFL - Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (1 Rue du Professeur Marion, 21000 Dijon - France)
- UNICANCER (101, rue de Tolbiac, 75654 Paris Cedex 13 - France)
6
Cancer Sciences Division (Southampton - United Kingdom)
- University of Southampton (University Road, Southampton SO17 1BJ - United Kingdom)
Abstract : We determined the number and functional status of CD4+ CD25(high) regulatory T cells (Treg) in blood samples from patients with metastatic carcinoma, and evaluated their sensitivity to a single intravenous infusion of cyclophosphamide. Treg numbers were significantly higher in 49 patients with metastatic cancer (9.2% of CD4+ T cells) compared to 24 healthy donors (7.1%). These cells expressed the transcription factor forkhead box P3 (FoxP3), glucocorticoid-induced tumour necrosis factor receptor family-related protein (GITR) and intracellular CD152, and demonstrated a suppressive activity in vitro against CD4+ CD25- autologous proliferation. At a single intravenous infusion, cyclophosphamide failed, in association with a non-specific immunotherapy by intratumoral bacille Calmette-Guérin (BCG), to modulate significantly Treg numbers or function. Metastatic cancer is associated with an expansion of peripheral blood CD4+ CD25(high) FoxP3+ GITR+ CD152+ Treg cells whose immunosuppressive properties do not differ from those of healthy subjects. Moreover, cyclophosphamide administration may not represent an optimal therapy to eliminate Treg, which further underlines the need to identify specific agents that would selectively deplete these cells.
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Journal articles
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https://www.hal.inserm.fr/inserm-00479739
Contributor : Philippe Saas <>
Submitted on : Sunday, May 2, 2010 - 2:28:06 PM
Last modification on : Wednesday, September 16, 2020 - 10:43:29 AM
Contributor : Philippe Saas <>
Submitted on : Sunday, May 2, 2010 - 2:28:06 PM
Last modification on : Wednesday, September 16, 2020 - 10:43:29 AM
Links full text
Identifiers
- HAL Id : inserm-00479739, version 1
- DOI : 10.1111/j.1365-2249.2007.03521.x
- PUBMED : 17956583
Collections
UNIV-FCOMTE | UMR645 | UNIV-BOURGOGNE | CGFL | FNCLCC | LNC-UMR866 | ENSBANA | AGROSUP-DIJON
Citation
S. Audia, A. Nicolas, D. Cathelin, N. Larmonier, Christophe Ferrand, et al.. Increase of CD4+ CD25+ regulatory T cells in the peripheral blood of patients with metastatic carcinoma: a Phase I clinical trial using cyclophosphamide and immunotherapy to eliminate CD4+ CD25+ T lymphocytes.. Clinical and Experimental Immunology, Wiley, 2007, 150 (3), pp.523-30. ⟨10.1111/j.1365-2249.2007.03521.x⟩. ⟨inserm-00479739⟩