Optimized vaccination regimen linked to exhaustive screening approaches identifies 2 novel HLA-B7 restricted epitopes within hepatitis C virus NS3 protein.

Perrine Martin; Peggy Parroche; Anthony Pajot; Laurence Chatel; Caroline Barreto; Leila Touat; Valérie Dubois; Pierre-Simon Rohrlich; Christine Bain; Christian Trépo; Francesco Negro; Geneviève Inchauspé; Anne Fournillier

doi:10.1016/j.micinf.2006.05.006

Optimized vaccination regimen linked to exhaustive screening approaches identifies 2 novel HLA-B7 restricted epitopes within hepatitis C virus NS3 protein.

Perrine Martin ^{1, 2} Peggy Parroche ^{1, 2} Anthony Pajot ³ Laurence Chatel ^{1, 2} Caroline Barreto ⁴ Leila Touat ^{1, 2} Valérie Dubois ⁵ Pierre-Simon Rohrlich ^{3, 6} Christine Bain ^{1, 2} Christian Trépo ⁷ Francesco Negro ⁸ Geneviève Inchauspé ^{1, 2} Anne Fournillier ^{1, 2}

1 IMIC - Immunothérapie des maladies infectieuses chroniques

2 BLG - BioSciences Lyon-Gerland

3 Immunité Cellulaire Antivirale

4 CIPF - Centre d'Immunologie Pierre Fabre

5 EFS Rhône-Alpes

6 HOTE GREFFON - Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098)

7 Service d'hépatologie

8 Division de Gastroentérologie, Hépatologie et Pathologie Clinique

Abstract : Broad immune responses, in particular specific for the NS3 protein and mediated by both CD8+ and CD4+T lymphocytes, are thought to play a critical role in the control of hepatitis C virus (HCV) infection. In this study, we searched for novel HLA-B*0702 NS3 restricted epitopes following an optimized NS3NS4 immunization protocol in transgenic mice expressing HLA-B*0702 molecule. Combining predicted and overlapping peptides, we identified two novel epitopes, WPA10 (aa 1111-1120) and LSP10 (aa 1153-1162), which triggered significant IFN-gamma-producing T cell frequencies and high CTL responses. Both epitopes were shown to be immunogenic when used as synthetic peptides to immunize mice. The relevance of these epitopes to humans was demonstrated, as both were able in vitro to recall specific IFN-gamma and IL10-producing cells from peripheral blood mononuclear cells of HCV infected patients. Such epitopes enlarge the pool of NS3-specific CD8+T cell epitopes available to perform immunomonitoring of HCV infection and to develop vaccines.

Type de document :

Article dans une revue

Microbes and Infection, Elsevier, 2006, 8 (9-10), pp.2432-41. 〈10.1016/j.micinf.2006.05.006〉

Domaine :

Sciences du Vivant [q-bio] / Immunologie

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https://www.hal.inserm.fr/inserm-00473470
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Soumis le : jeudi 15 avril 2010 - 11:59:57
Dernière modification le : lundi 9 juillet 2018 - 13:12:02

Optimized vaccination regimen linked to exhaustive screening approaches identifies 2 novel HLA-B7 restricted epitopes within hepatitis C virus NS3 protein.

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Optimized vaccination regimen linked to exhaustive screening approaches identifies 2 novel HLA-B7 restricted epitopes within hepatitis C virus NS3 protein.

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