Differential sensitivity of T lymphocytes and hematopoietic precursor cells to photochemotherapy with 8-methoxypsoralen and ultraviolet A light.

Mohamed Mabed 1 Christian Coffe 2 Evelyne Racadot 2 Regis Angonin 2 Jean-Jaques Pavey 3 Pierre Tiberghien 4 Patrick Herve 4
1 Hematology and Medical Oncology Unit
2 Laboratoire d'anatomie pathologique [Besancon]
3 Service de radiothérapie
4 HOTE GREFFON - Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098)
Abstract : The combination of 8-methoxypsoralen (8-MOP) and long wave ultraviolet radiation (UV-A) has immunomodulatory effects and might abolish both graft-vs-host and host-vs-graft reactions after allogeneic hematopoietic stem cell transplantation. In the present study, we have confirmed the sensitivity of T lymphocytes to 8-MOP treatment plus UV-A exposure as evidenced by the abrogation of the alloreactivity in mixed lymphocyte cultures as well as the inhibition of the response to phytohemagglutinin A. However, the clonogenic capacity of the bone marrow hematopoietic progenitors was inhibited with UV-A doses lower than the doses needed to inhibit T-lymphocytes alloreactivity. Moreover, long-term bone marrow cultures showed that 8-MOP plus UV-A treatment had detrimental effects on the more immature bone marrow stem cells. These data were confirmed when murine bone marrow graft was treated with 8-MOP, exposed to UV-A, then transplanted into semiallogeneic recipient mice. The treated cells could not maintain their clonogenic capacity in vivo resulting in death of all animals. Taken together, these data show that ex vivo 8-MOP plus UV-A treatment of the marrow graft cannot be used to prevent post-bone marrow transplantation alloreactivity.
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Annals of Hematology, Springer Verlag, 2006, 85 (1), pp.17-24. 〈10.1007/s00277-005-0009-9〉
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Contributeur : Philippe Saas <>
Soumis le : jeudi 15 avril 2010 - 11:23:07
Dernière modification le : lundi 9 juillet 2018 - 13:12:02

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Mohamed Mabed, Christian Coffe, Evelyne Racadot, Regis Angonin, Jean-Jaques Pavey, et al.. Differential sensitivity of T lymphocytes and hematopoietic precursor cells to photochemotherapy with 8-methoxypsoralen and ultraviolet A light.. Annals of Hematology, Springer Verlag, 2006, 85 (1), pp.17-24. 〈10.1007/s00277-005-0009-9〉. 〈inserm-00473407〉

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