Comparison of ultraviolet B-induced imbalance of antioxidant status in foreskin- and abdominal skin-derived human fibroblasts.

Thibaut Saguet 1, 2 Sophie Robin 3 Laurence Nicod 4 Delphine Binda 1, 2 Céline Viennet 1, 2 François Aubin 1, 5 Bernard Coulomb 6 Philippe Humbert 1, 2, 7
1 LIBC - Laboratoire d'Ingénierie et Biologie Cutanées
2 HOTE GREFFON - Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098)
3 BioExigence
4 Sciences séparatives et biopharmaceutiques
5 CEF2P / CARCINO - Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181)
6 Réparation et remodelage oro-fasciaux
7 Département de dermatologie
Abstract : Ultraviolet B radiation (UVB) is involved in the development of deleterious cutaneous damage. Several changes could be attributed to UVB-induced reactive oxygen species attacks in fibroblasts. However dermal cells from young and adult skin could respond differently to oxidative stress. So antioxidant status and its consequences on cytotoxicity and apoptosis were compared in child foreskin fibroblasts (FF) and adult abdominal skin fibroblasts (AF) in response to UVB. Basal levels of lipid peroxidation tended to be higher in AF than in FF, which could be related to a reshaping of antioxidant defences (higher catalase and lower superoxide dismutase activities). AF and FF appeared to react similarly to high UVB doses as regards cytotoxicity and apoptosis which increased significantly 24h after exposure. The enhancement of cell death could be due to the inherent oxidative stress: glutathione appeared significantly decreased in both cell populations. As a consequence AF, but not FF, presented significantly increased levels of lipid peroxidation, which could be explained by the pre-cited differences of basal antioxidant defences. These results suggest that AF and FF do not respond to UVB by the same pathway.
Type de document :
Article dans une revue
european journal of dermatology : EJD, 2006, 16 (4), pp.368-74
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http://www.hal.inserm.fr/inserm-00473366
Contributeur : Philippe Saas <>
Soumis le : jeudi 15 avril 2010 - 10:57:00
Dernière modification le : mercredi 5 septembre 2018 - 17:04:04

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  • HAL Id : inserm-00473366, version 1
  • PUBMED : 16935792

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UNIV-FCOMTE | UMR645 | UNIV-PARIS5 | USPC

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Thibaut Saguet, Sophie Robin, Laurence Nicod, Delphine Binda, Céline Viennet, et al.. Comparison of ultraviolet B-induced imbalance of antioxidant status in foreskin- and abdominal skin-derived human fibroblasts.. european journal of dermatology : EJD, 2006, 16 (4), pp.368-74. 〈inserm-00473366〉

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