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Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.

Abstract : Cell cycle dynamics and localization of condensins--multiprotein complexes involved in late stages of mitotic chromosome condensation--were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to beta-tubulin levels remains almost constant from G1 to M phase. pEg7 and XCAP-E were localized to the mitotic chromosomes and were detected in interphase nuclei. Immunostaining for condensins and nucleolar proteins UBF, fibrillarin and B23 revealed that both XCAP-E and pEg7 are localized in the granular component of the nucleolus. Nucleolar labeling of both proteins is preserved in segregated nucleoli after 6 hours of incubation with actinomycin D (5 mg/ml), but the size of the labeled zone was significantly smaller. The data suggest a novel interphase function of condensin subunits in spatial organization of the nucleolus and/or ribosome biogenesis.
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https://www.hal.inserm.fr/inserm-00467029
Contributor : Vincent Legagneux <>
Submitted on : Thursday, March 25, 2010 - 4:30:36 PM
Last modification on : Thursday, August 1, 2019 - 2:13:44 PM

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Rustem Uzbekov, Elmira Timirbulatova, Erwan Watrin, Fabien Cubizolles, David Ogereau, et al.. Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.. Journal of Cell Science, Company of Biologists, 2003, 116 (Pt 9), pp.1667-78. ⟨10.1242/jcs.00311⟩. ⟨inserm-00467029⟩

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