Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.

Rustem Uzbekov; Elmira Timirbulatova; Erwan Watrin; Fabien Cubizolles; David Ogereau; Pavel Gulak; Vincent Legagneux; Vladimir Ju Polyakov; Katherine Le Guellec; Igor Kireev

doi:10.1242/jcs.00311

Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.

Rustem Uzbekov ^{1, 2} Elmira Timirbulatova ¹ Erwan Watrin ² Fabien Cubizolles ² David Ogereau ² Pavel Gulak ³ Vincent Legagneux ² Vladimir Ju Polyakov ^{2, 4} Katherine Le Guellec ² Igor Kireev ^{1, 5}

1 Cell Cycle Group, Division of Electron Microscopy

2 Génétique et Développement

3 Institute of Agricultural Biotechnology

4 Division of Electron Microscopy

5 Department of Cell and Structural Biology

Abstract : Cell cycle dynamics and localization of condensins--multiprotein complexes involved in late stages of mitotic chromosome condensation--were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to beta-tubulin levels remains almost constant from G1 to M phase. pEg7 and XCAP-E were localized to the mitotic chromosomes and were detected in interphase nuclei. Immunostaining for condensins and nucleolar proteins UBF, fibrillarin and B23 revealed that both XCAP-E and pEg7 are localized in the granular component of the nucleolus. Nucleolar labeling of both proteins is preserved in segregated nucleoli after 6 hours of incubation with actinomycin D (5 mg/ml), but the size of the labeled zone was significantly smaller. The data suggest a novel interphase function of condensin subunits in spatial organization of the nucleolus and/or ribosome biogenesis.

Type de document :

Article dans une revue

Journal of Cell Science, Company of Biologists, 2003, 116 (Pt 9), pp.1667-78. 〈10.1242/jcs.00311 〉

Domaine :

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire / Génomique, Transcriptomique et Protéomique [q-bio.GN]

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https://www.hal.inserm.fr/inserm-00467029
Contributeur : Vincent Legagneux <>
Soumis le : jeudi 25 mars 2010 - 16:30:36
Dernière modification le : mercredi 16 mai 2018 - 11:23:33

Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.

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Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.

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