Skip to Main content Skip to Navigation
Journal articles

Distribution of XCAP-E and XCAP-D2 in the Xenopus oocyte nucleus.

Abstract : Several antibodies were used to examine the distribution of two condensin members, XCAP-E and XCAP-D2, in the nucleus of Xenopus oocytes. XCAP-D2 was found to be associated with the lampbrush chromosomes. The chromosomal regions containing XCAP-D2 correspond precisely to domains of highly compacted chromatin, suggesting a direct contribution of XCAP-D2 in meiotic chromatin organization. In contrast, XCAP-E was found to be absent from chromosomes but was detected at a high concentration in the granular component of nucleoli. The subnucleolar localization of XCAP-E was further confirmed by double labeling using several nucleolar protein markers. The fate of nucleolar XCAP-E was also followed when changes in the nucleoli morphology were artificially induced. The apparent exclusion of XCAP-E from the ribosomal DNA and its tight association with the granular component in all preparations suggest that it might be sequestrated in nucleoli during early stages of meiosis. Interestingly, both XCAP-D2 and XCAP-E were also detected in Cajal bodies, which are organelles suspected to play a role in the assembly/modification of the RNA transcription and processing machinery. The presence of two condensins in CBs might extend such a role of assembly to chromatin macromolecular components as well.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00466995
Contributor : Vincent Legagneux <>
Submitted on : Thursday, March 25, 2010 - 3:10:45 PM
Last modification on : Thursday, August 1, 2019 - 2:13:44 PM

Links full text

Identifiers

Citation

Brent Beenders, Erwan Watrin, Vincent Legagneux, Igor Kireev, Michel Bellini. Distribution of XCAP-E and XCAP-D2 in the Xenopus oocyte nucleus.. Chromosome Research, Springer Verlag, 2003, 11 (6), pp.549-64. ⟨10.1023/A:1024999316867⟩. ⟨inserm-00466995⟩

Share

Metrics

Record views

274