The Motor Protein Myosin-X Transports VE-Cadherin along Filopodia To Allow the Formation of Early Endothelial Cell-Cell Contacts

Vascular endothelium (VE), the monolayer of endothelial cells that lines the vascular tree, undergoes damage at the basis of some vascular diseases. Its integrity is maintained by VE-cadherin, an adhesive receptor localized at cell-cell junctions. Here, we show that VE-cadherin is also located at the tip and along filopodia in sparse or subconfluent endothelial cells. We observed that VE-cadherin navigates along intrafilopodial actin filaments. We found that the actin motor protein myosin-X is colocalized and moves synchronously with filopodial VE-cadherin. Immunoprecipitation and pulldown assays confirmed that myosin-X is directly associated with the VE-cadherin complex. Furthermore, expression of a dominant-negative mutant of myosin-X revealed that myosin-X is required for VE-cadherin export to cell edges and filopodia. These features indicate that myosin-X establishes a link between the actin cytoskeleton and VE-cadherin, thereby allowing VEcadherin transportation along intrafilopodial actin cables. In conclusion, we propose that VE-cadherin trafficking along filopodia using myosin-X motor protein is a prerequisite for cell-cell junction formation. This mechanism may have functional consequences for endothelium repair in pathological settings.

Keywords

VE-cadherin Myosin-X actin cytoskeleton filopodia transport videomicroscopy

Domains

Life Sciences [q-bio] Cellular Biology

Fichier principal

Version_pdf_MCB_2010-01-20.pdf (26.88 Mo)

Movie_1.mov (444.59 Ko)

Movie_10.mov (7.55 Mo)

Movie_2.mov (371.97 Ko)

Movie_3.mov (3.63 Mo)

Movie_4.mov (1.58 Mo)

Movie_5.mov (3.72 Mo)

Movie_6.mov (4.38 Mo)

Movie_7.mov (651.25 Ko)

Movie_8.mov (3.89 Mo)

Movie_9.mov (4.75 Mo)

Origin : Files produced by the author(s)

Format : Other

Danielle Gulino-Debrac : Connect in order to contact the contributor

https://www.hal.inserm.fr/inserm-00462307

Submitted on : Tuesday, March 9, 2010-10:53:06 AM

Last modification on : Friday, October 14, 2022-4:05:26 AM

Long-term archiving on: Friday, June 18, 2010-7:15:53 PM

Dates and versions

inserm-00462307 , version 1 (09-03-2010)

Identifiers

HAL Id : inserm-00462307 , version 1
DOI : 10.1128/MCB.01226-09
PUBMED : 20123970

Cite

Sébastien Almagro, Claire Durmort, Adeline Chervin-Pétinot, Stéphanie Heyraud, Mathilde Dubois, et al.. The Motor Protein Myosin-X Transports VE-Cadherin along Filopodia To Allow the Formation of Early Endothelial Cell-Cell Contacts: MYOSIN-X TRANSPORT OF VE-CADHERIN ALONG FILOPODIA. Molecular and Cellular Biology, 2010, Apr. 2010, page 1703-1717. ⟨10.1128/MCB.01226-09⟩. ⟨inserm-00462307⟩

Export

BibTeX TEI Dublin Core DC Terms EndNote Datacite

Collections

INSERM CEA UGA CNRS IBS IBS-MEM INC-CNRS CEA-DRF IRIG CEA-GRE ANR

300 View

258 Download