Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions.

Christian Villiers 1, * Heidi Freitas 1 Rachel Couderc 1 Marie-Bernadette Villiers 1 Patrice Marche 2
* Auteur correspondant
1 INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
2 INSERM U823, équipe 8 (Immunologie Analytique des Pathologies Chroniques)
INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
Abstract : The effect of manufactured gold nanoparticles (NP) on the immune system was analysed through their ability to perturb the functions of dendritic cells (DC), a major actor of both innate and acquired immune responses. For this purpose, DCs were produced in culture from mouse bone marrow progenitors.The analysis of the viability of the cells after their incubation in the presence of gold NP shows that these NP are not cytotoxics even at high concentration. Furthermore, the phenotype of the DC is unchanged after the addition of NP, indicating that there is no activation of the DC. But the analysis of the cells at the intracellular level reveals important amounts of gold NP amassing in endocytic compartments. Furthermore, the secretion of cytokines is significantly modified after such internalisation indicating a potential perturbation of the immune response.
Keywords : cytokine Nanoparticles gold immune system dendritic cells cytotoxicity cytokine.
Type de document :
Article dans une revue
Journal of Nanoparticle Research, Springer Verlag, 2010, 12 (1), pp.55-60. 〈10.1007/s11051-009-9692-0〉
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Christian Villiers, Heidi Freitas, Rachel Couderc, Marie-Bernadette Villiers, Patrice Marche. Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions.. Journal of Nanoparticle Research, Springer Verlag, 2010, 12 (1), pp.55-60. 〈10.1007/s11051-009-9692-0〉. 〈inserm-00458282〉

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