 |
Journal articles
A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates.
Guillaume Blin
1
David Nury
1
Sonia Stefanovic
1
Tui Neri
1
Orianne Guillevic
1
Benjamin Brinon
1, 2
Valérie Bellamy
2
Catherine Rücker-Martin
3
Pascal Barbry
4
Alain Bel
5
Lionel Bonnevie
6
Patrick Bruneval
7
Chad Cowan
8
Julia Pouly
5
Shoukhrat Mitalipov
9
Elodie Gouadon
3
Patrice Binder
10
Albert Hagège
2, 11
Michel Desnos
2, 11
Philippe Menasché
2, 5, *
Jean-François Renaud
3
Michel Puceat
1, 12, *
*
Corresponding author
Guillaume Blin 1
Author
David Nury 1
Author
Sonia Stefanovic 1
Author IdHAL : sonia-stefanovic ORCID : https://orcid.org/0000-0003-4202-2534
Tui Neri 1
Author
Orianne Guillevic 1
Author
Benjamin Brinon 1, 2
Author
Valérie Bellamy 2
Author
Catherine Rücker-Martin 3
Author
Patrice Binder 10
Author
Albert A. Hagège 2, 11
Author
Michel Desnos 2, 11
Author
Philippe Menasché 2, 5
Correspondent author
Jean-François Renaud 3
Author
Michel Puceat 1, 12
Correspondent author IdHAL : michel-puceat ResearcherId : http://www.researcherid.com/rid/ R-6140-2017 ORCID : https://orcid.org/0000-0001-9055-7563
1
I-STEM - Institut des cellules souches pour le traitement et l'étude des maladies monogéniques (GENETHON 1, rue de l'internationale BP 118 94004 EVRY CEDEX - France)
- UEVE - Université d'Évry-Val-d'Essonne (Boulevard François Mitterrand 91025 Evry Cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U861 (101, rue de Tolbiac, 75013 Paris - France)
2
UMR_S 633 - Therapie Cellulaire en Pathologie Cardio-Vasculaire (Hopital Broussais PARIS V 96, Rue Didot 75014 PARIS - France)
- UPD5 - Université Paris Descartes - Paris 5 : UMR_S 633 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U633 (101, rue de Tolbiac, 75013 Paris - France)
3
RTFSPP - Remodelage tissulaire et fonctionnel : signalisation et physio-pathologie (CNRS UMR 8162 - Bât. Recherche 133 Avenue de la Résistance 92350 LE PLESSIS ROBINSON - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- Centre chirurgical Marie Lannelongue (France)
- CNRS - Centre National de la Recherche Scientifique : UMR8162 (France)
4
IPMC - Institut de pharmacologie moléculaire et cellulaire (CNRS-IPMC 660 Route des lucioles 06560 VALBONNE - France)
- UNS - Université Nice Sophia Antipolis (... - 2019) (Parc Valrose - BP 2135 - 06103 Nice cedex 2 - France)
- COMUE UCA - COMUE Université Côte d'Azur (2015 - 2019) (Parc Valrose, 28 avenue Valrose 06108 Nice Cedex 2 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR6097 (France)
5
Service de chirurgie cardiaque et vasculaire [CHU HEGP] (CHU HEGP 20, rue Leblanc 75908 Paris Cedex 15 - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- HEGP - Hôpital Européen Georges Pompidou [APHP] (20, rue Leblanc, 75015 Paris - France)
7
Service d'anatomo-pathologie [CHU HEGP] (20 Rue Leblanc, 75015 Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- HEGP - Hôpital Européen Georges Pompidou [APHP] (20, rue Leblanc, 75015 Paris - France)
8
Stowers Medical Institute (Center for Regenerative Medicine and Technology, Boston - United States)
- Cardiovascular Research Center (France)
9
Division of Reproductive Sciences (Beaverton, OR - United States)
- ONPRC - Oregon National Primate Research Center (505 NW 185th Ave, Beaverton, OR 97006, États-Unis - United States)
- OHSU - Oregon Health and Science University [Portland] (3181 S.W. Sam Jackson Park Rd. Portland, Oregon 97239-3098 - United States)
10
IMASSA - Institut de Médecine Aérospatiale du Service de Santé des Armées (B.P.73, 91223 Brétigny-sur-Orge Cedex - France)
- Service de Santé des Armées (France)
11
Service de cardiologie [CHU HEGP] (20 rue Leblanc, Cedex 15, 75908 Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- HEGP - Hôpital Européen Georges Pompidou [APHP] (20, rue Leblanc, 75015 Paris - France)
12
Equipe avenir HUMAN EMBRYONIC STEM CELLS AS A MODEL TO DELINEATE THE EARLY CARDIAC TRANSCRIPTIONAL NETWORKS: TOWARD A BETTER UNDERSTANDING OF CARDIAC CONGENITAL AND GENETIC DISEASES. (4 rue piere fontaine Campus genopole 1 91058 evry - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U633 (101, rue de Tolbiac, 75013 Paris - France)
Abstract : Cell therapy holds promise for tissue regeneration, including in individuals with advanced heart failure. However, treatment of heart disease with bone marrow cells and skeletal muscle progenitors has had only marginal positive benefits in clinical trials, perhaps because adult stem cells have limited plasticity. The identification, among human pluripotent stem cells, of early cardiovascular cell progenitors required for the development of the first cardiac lineage would shed light on human cardiogenesis and might pave the way for cell therapy for cardiac degenerative diseases. Here, we report the isolation of an early population of cardiovascular progenitors, characterized by expression of OCT4, stage-specific embryonic antigen 1 (SSEA-1), and mesoderm posterior 1 (MESP1), derived from human pluripotent stem cells treated with the cardiogenic morphogen BMP2. This progenitor population was multipotential and able to generate cardiomyocytes as well as smooth muscle and endothelial cells. When transplanted into the infarcted myocardium of immunosuppressed nonhuman primates, an SSEA-1+ progenitor population derived from Rhesus embryonic stem cells differentiated into ventricular myocytes and reconstituted 20% of the scar tissue. Notably, primates transplanted with an unpurified population of cardiac-committed cells, which included SSEA-1- cells, developed teratomas in the scar tissue, whereas those transplanted with purified SSEA-1+ cells did not. We therefore believe that the SSEA-1+ progenitors that we have described here have the potential to be used in cardiac regenerative medicine.
Document type :
Journal articles
Complete list of metadatas
https://www.hal.inserm.fr/inserm-00451770
Contributor : Michel Puceat <>
Submitted on : Monday, March 29, 2010 - 8:49:31 AM
Last modification on : Wednesday, September 16, 2020 - 5:04:55 PM
Long-term archiving on: : Tuesday, September 28, 2010 - 12:07:50 PM
Contributor : Michel Puceat <>
Submitted on : Monday, March 29, 2010 - 8:49:31 AM
Last modification on : Wednesday, September 16, 2020 - 5:04:55 PM
Long-term archiving on: : Tuesday, September 28, 2010 - 12:07:50 PM
File
BlinNuryStefanovic_et_al_JCIac...
Files produced by the author(s)
Identifiers
- HAL Id : inserm-00451770, version 1
- DOI : 10.1172/JCI40120
- PUBMED : 20335662
Collections
UNICE | SSA | CNRS | UNIV-EVRY | UNIV-PARIS5 | USPC | I-STEM | UNIV-COTEDAZUR
Citation
Guillaume Blin, David Nury, Sonia Stefanovic, Tui Neri, Orianne Guillevic, et al.. A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates.. Journal of Clinical Investigation, American Society for Clinical Investigation, 2010, 120 (4), pp.1125-39. ⟨10.1172/JCI40120⟩. ⟨inserm-00451770⟩
Metrics
Record views
1757
Files downloads