Skip to Main content Skip to Navigation
Journal articles

In vivo silencing of Reptin blocks the progression of human hepatocellular carcinoma in xenografts and is associated with replicative senescence.: Reptin silencing blocks liver cancer progression

Abstract : BACKGROUND & AIMS: We previously showed that Reptin is overexpressed in hepatocellular carcinoma (HCC), and that in vitro depletion of Reptin with siRNAs led to HCC cell growth arrest and apoptosis. Here, we asked whether in vivo targeting of Reptin in established tumours had a therapeutic effect. METHODS: We used lentiviral vectors to construct HuH7 and Hep3B cell lines with doxycycline (Dox)-dependent expression of Reptin (R2) or control shRNA (GL2). Cells were injected subcutaneously into immunodeficient mice, and Dox was given when tumours reached a volume of 250 mm(3). RESULTS: In vitro, the growth of GL2-Dox, GL2+Dox, and R2-Dox cells was undistinguishable whereas that of R2+Dox cells stopped 4 days after Dox treatment. The growth decrease was associated with increased apoptosis, and evidence of replicative senescence, as shown by staining for acid beta-galactosidase and the presence of senescence-associated heterochromatin foci. In xenografted mice, R2+Dox tumour growth stagnated or even regressed with prolonged treatment in contrast with the GL2-Dox, GL2+Dox, and R2-Dox tumours that progressed steadily. The blockage of tumour progression was associated with the induction of senescence and reduced cell proliferation. CONCLUSIONS: In vivo Reptin depletion leads to tumour growth arrest. Reptin may prove a valuable target in HCC.
Document type :
Journal articles
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00449372
Contributor : Jean Rosenbaum <>
Submitted on : Thursday, January 21, 2010 - 2:23:02 PM
Last modification on : Thursday, June 11, 2020 - 3:19:41 AM
Long-term archiving on: : Friday, June 18, 2010 - 1:14:03 AM

File

Menard.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Ludovic Ménard, Danièle Taras, Aude Grigoletto, Valérie Haurie, Alexandra Nicou, et al.. In vivo silencing of Reptin blocks the progression of human hepatocellular carcinoma in xenografts and is associated with replicative senescence.: Reptin silencing blocks liver cancer progression. Journal of Hepatology, Elsevier, 2010, 52 (5), pp.681-9. ⟨10.1016/j.jhep.2009.12.029⟩. ⟨inserm-00449372⟩

Share

Metrics

Record views

499

Files downloads

1214