An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization.

Michel Jourdan 1 Anouk Caraux 1 John De Vos 1, 2 Geneviève Fiol 1 Marion Larroque 1 Chantal Cognot 3 Caroline Bret 1 Christophe Duperray 1 Dirk Hose 4, 5 Bernard Klein 1, 2, *
* Auteur correspondant
1 IRB - Institut de recherche en biothérapie
2 UM1 Médecine - Montpellier 1 - UFR de Médecine
3 Laboratoire d'immunologie
4 Department of Internal Medicine V
5 Nationales Centrum für Tumorerkrankungen
Abstract : Human plasma cells (PCs) and their precursors play an essential role in humoral immune response but are rare and difficult to harvest. We report the generation of human syndecan-1(+) and immunoglobulin secreting PCs starting from memory B cells in a 3-step and 10-day (D) culture, including a 6-fold cell amplification. We report the detailed phenotypic and Affymetrix gene expression profiles of these in vitro PCs as well as of intermediate cells (activated B cells and plasmablasts) compared with memory B cells and bone marrow PCs, which is accessible through an open web ATLAS (http://amazonia.transcriptome.eu/). We show this B cell-to-PC differentiation to involve IRF4 and AICDA expressions in D4 activated B cells, decrease of PAX5 and BCL6 expressions, and increase in PRDM1 and XBP1 expressions in D7 plasmablasts and D10 PCs. It involves down-regulation of genes controlled by Pax5 and induction of genes controlled by Blimp-1 and XBP1 (unfold protein response). The detailed phenotype of D10 PCs resembles that of peripheral blood PCs detected after immunization of healthy donors. This in vitro model will facilitate further studies in PC biology. It will likewise be helpful to study PC dyscrasias, including multiple myeloma.
Keywords : plasma cell B lymphocyte transcription factors multiple myeloma
Type de document :
Article dans une revue
Blood, American Society of Hematology, 2009, 114 (25), pp.5173-81. 〈10.1182/blood-2009-07-235960〉
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Soumis le : lundi 25 janvier 2010 - 13:57:50
Dernière modification le : vendredi 19 octobre 2018 - 19:30:03
Document(s) archivé(s) le : jeudi 18 octobre 2012 - 12:15:58

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Michel Jourdan, Anouk Caraux, John De Vos, Geneviève Fiol, Marion Larroque, et al.. An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization.. Blood, American Society of Hematology, 2009, 114 (25), pp.5173-81. 〈10.1182/blood-2009-07-235960〉. 〈inserm-00446133〉

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