RNAi-mediated targeting of heterochromatin by the RITS complex.

André Verdel 1 Songtao Jia 2 Scott Gerber 1, 3 Tomoyasu Sugiyama 2 Steven Gygi 1, 3 Shiv Grewal 2 Danesh Moazed 1
1 Department of cell biology
2 Laboratory of Molecular Cell Biology
3 Taplin Biological Mass Spectrometry Facility
Abstract : RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast. The RITS complex contains Ago1 (the fission yeast Argonaute homolog), Chp1 (a heterochromatin-associated chromodomain protein), and Tas3 (a novel protein). In addition, the complex contains small RNAs that require the Dicer ribonuclease for their production. These small RNAs are homologous to centromeric repeats and are required for the localization of RITS to heterochromatic domains. The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci.
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Science, American Association for the Advancement of Science, 2004, 303 (5658), pp.672-6. 〈10.1126/science.1093686〉
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http://www.hal.inserm.fr/inserm-00444432
Contributeur : Andre Verdel <>
Soumis le : mercredi 6 janvier 2010 - 15:43:21
Dernière modification le : vendredi 30 mars 2018 - 16:00:02

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André Verdel, Songtao Jia, Scott Gerber, Tomoyasu Sugiyama, Steven Gygi, et al.. RNAi-mediated targeting of heterochromatin by the RITS complex.. Science, American Association for the Advancement of Science, 2004, 303 (5658), pp.672-6. 〈10.1126/science.1093686〉. 〈inserm-00444432〉

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