Skip to Main content Skip to Navigation
Journal articles

Orientation of Mitotic Spindles during the 8- to 16-Cell Stage Transition in Mouse Embryos.

Abstract : BACKGROUND: Asymmetric cell divisions are involved in the divergence of the first two lineages of the pre-implantation mouse embryo. They first take place after cell polarization (during compaction) at the 8-cell stage. It is thought that, in contrast to many species, spindle orientation is random, although there is no direct evidence for this. METHODOLOGY/PRINCIPAL FINDINGS: Tubulin-GFP and live imaging with a spinning disk confocal microscope were used to directly study spindle orientation in whole embryos undergoing the 8- to 16-cell stage transition. This approach allowed us to determine that there is no predetermined cleavage pattern in 8-cell compacted mouse embryos and that mitotic spindle orientation in live embryo is only modulated by the extent of cell rounding up during mitosis. CONCLUSIONS: These results clearly demonstrate that spindle orientation is not controlled at the 8- to 16-cell transition, but influenced by cell bulging during mitosis, thus reinforcing the idea that pre-implantation development is highly regulative and not pre-patterned.
Document type :
Journal articles
Complete list of metadata

Cited literature [15 references]  Display  Hide  Download
Contributor : Hervé de Villemeur <>
Submitted on : Monday, December 14, 2009 - 9:30:59 AM
Last modification on : Wednesday, December 9, 2020 - 3:45:11 AM
Long-term archiving on: : Thursday, June 17, 2010 - 8:46:49 PM


Publisher files allowed on an open archive



Nicolas Dard, Sophie Louvet-Vallée, Bernard Maro. Orientation of Mitotic Spindles during the 8- to 16-Cell Stage Transition in Mouse Embryos.. PLoS ONE, Public Library of Science, 2009, 4 (12), pp.e8171. ⟨10.1371/journal.pone.0008171⟩. ⟨inserm-00440937⟩



Record views


Files downloads