Orientation of Mitotic Spindles during the 8- to 16-Cell Stage Transition in Mouse Embryos.

Abstract : BACKGROUND: Asymmetric cell divisions are involved in the divergence of the first two lineages of the pre-implantation mouse embryo. They first take place after cell polarization (during compaction) at the 8-cell stage. It is thought that, in contrast to many species, spindle orientation is random, although there is no direct evidence for this. METHODOLOGY/PRINCIPAL FINDINGS: Tubulin-GFP and live imaging with a spinning disk confocal microscope were used to directly study spindle orientation in whole embryos undergoing the 8- to 16-cell stage transition. This approach allowed us to determine that there is no predetermined cleavage pattern in 8-cell compacted mouse embryos and that mitotic spindle orientation in live embryo is only modulated by the extent of cell rounding up during mitosis. CONCLUSIONS: These results clearly demonstrate that spindle orientation is not controlled at the 8- to 16-cell transition, but influenced by cell bulging during mitosis, thus reinforcing the idea that pre-implantation development is highly regulative and not pre-patterned.
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PLoS ONE, Public Library of Science, 2009, 4 (12), pp.e8171. 〈10.1371/journal.pone.0008171〉
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Nicolas Dard, Sophie Louvet-Vallée, Bernard Maro. Orientation of Mitotic Spindles during the 8- to 16-Cell Stage Transition in Mouse Embryos.. PLoS ONE, Public Library of Science, 2009, 4 (12), pp.e8171. 〈10.1371/journal.pone.0008171〉. 〈inserm-00440937〉

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