SUMOylation of human peroxisome proliferator-activated receptor alpha inhibits its trans-activity through the recruitment of the nuclear corepressor NCoR. - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 2010

SUMOylation of human peroxisome proliferator-activated receptor alpha inhibits its trans-activity through the recruitment of the nuclear corepressor NCoR.

(1) , (2) , (1) , (1) , (1)
1
2

Abstract

The nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha) is a key regulator of genes implicated in lipid homeostasis and inflammation. PPARalpha trans-activity is enhanced by recruitment of coactivators such as SRC1 and CBP/p300 and is inhibited by binding of corepressors such as NCoR and SMRT. In addition to ligand binding, PPARalpha activity is regulated by post-translational modifications such as phosphorylation and ubiquitination. In this report, we demonstrate that hPPARalpha is SUMOylated by SUMO-1 on lysine 185 in the hinge region. The E2-conjugating enzyme Ubc9 and the SUMO E3- ligase PIASy are implicated in this process. In addition, ligand treatment decreases the SUMOylation rate of hPPARalpha. Finally, our results demonstrate that SUMO-1 modification of hPPARalpha down-regulates its trans-activity through the specific recruitment of corepressor NCoR but not SMRT leading to the differential expression of a subset of PPARalpha target genes. In conclusion, hPPARalpha SUMOylation on lysine 185 down-regulates its trans-activity through the selective recruitment of NCoR.
Fichier principal
Vignette du fichier
inserm-00439808_edited.pdf (693.76 Ko) Télécharger le fichier
Vignette du fichier
JBC-2009-Glineur.pdf (3.45 Mo) Télécharger le fichier
Vignette du fichier
Supplementary_data_JBC-2009.pdf (61.97 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Loading...

Dates and versions

inserm-00439808 , version 1 (15-12-2010)

Identifiers

Cite

Benoit Pourcet, Inés Pineda-Torra, Bruno Derudas, Bart Staels, Corine Glineur. SUMOylation of human peroxisome proliferator-activated receptor alpha inhibits its trans-activity through the recruitment of the nuclear corepressor NCoR.. Journal of Biological Chemistry, 2010, 285 (9), pp.5983-92. ⟨10.1074/jbc.M109.078311⟩. ⟨inserm-00439808⟩
124 View
585 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More