TAF4/4b x TAF12 displays a unique mode of DNA binding and is required for core promoter function of a subset of genes.

Kfir Gazit 1 Sandra Moshonov 1 Rofa Elfakess 1 Michal Sharon 1 Gabrielle Mengus 2 Irwin Davidson 2 Rivka Dikstein 1
1 Department of Biological Chemistry
2 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
Abstract : The major core promoter-binding factor in polymerase II transcription machinery is TFIID, a complex consisting of TBP, the TATA box-binding protein, and 13 to 14 TBP-associated factors (TAFs). Previously we found that the histone H2A-like TAF paralogs TAF4 and TAF4b possess DNA-binding activity. Whether TAF4/TAF4b DNA binding directs TFIID to a specific core promoter element or facilitates TFIID binding to established core promoter elements is not known. Here we analyzed the mode of TAF4b.TAF12 DNA binding and show that this complex binds DNA with high affinity. The DNA length required for optimal binding is approximately 70 bp. Although the complex displays a weak sequence preference, the nucleotide composition is less important than the length of the DNA for high affinity binding. Comparative expression profiling of wild-type and a DNA-binding mutant of TAF4 revealed common core promoter features in the down-regulated genes that include a TATA-box and an Initiator. Further examination of the PEL98 gene from this group showed diminished Initiator activity and TFIID occupancy in TAF4 DNA-binding mutant cells. These findings suggest that DNA binding by TAF4/4b-TAF12 facilitates the association of TFIID with the core promoter of a subset of genes.
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Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (39), pp.26286-96. 〈10.1074/jbc.M109.011486〉
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Soumis le : vendredi 4 décembre 2009 - 11:39:47
Dernière modification le : mercredi 14 mars 2018 - 16:47:08

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Kfir Gazit, Sandra Moshonov, Rofa Elfakess, Michal Sharon, Gabrielle Mengus, et al.. TAF4/4b x TAF12 displays a unique mode of DNA binding and is required for core promoter function of a subset of genes.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (39), pp.26286-96. 〈10.1074/jbc.M109.011486〉. 〈inserm-00438643〉

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