miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice.

Cyrielle Clapé 1 Vanessa Fritz 1 Corinne Henriquet 1 Florence Apparailly 2 Pedro Luis Fernandez 3, 4 François Iborra 5 Christophe Avancès 6, 7 Martin Villalba 8 Stéphane Culine 9 Lluis Fajas 1, 5, *
* Auteur correspondant
1 IRCM - U896 Inserm - UM1 - Institut de recherche en cancérologie de Montpellier
2 Cellules souches mésenchymateuses, environnement articulaire et immunothérapies de la polyarthrite rhumatoide
3 IDIBAPS - Institut d'Investigacions Biomèdiques August Pi i Sunyer
4 Department of pathology
5 Service d'urologie
6 Service d'urologie
7 Service d'urologie
8 IGMM - Institut de Génétique Moléculaire de Montpellier
9 Départment d'oncologie médicale
Abstract : BACKGROUND: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. RESULTS: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. CONCLUSIONS: miR-143 is as a new target for prostate cancer treatment.
keyword : ERK5 miR-143 prostate cancer ERK5.
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PLoS ONE, Public Library of Science, 2009, 4 (10), pp.e7542. 〈10.1371/journal.pone.0007542〉
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Cyrielle Clapé, Vanessa Fritz, Corinne Henriquet, Florence Apparailly, Pedro Luis Fernandez, et al.. miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice.. PLoS ONE, Public Library of Science, 2009, 4 (10), pp.e7542. 〈10.1371/journal.pone.0007542〉. 〈inserm-00431462〉

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