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miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice.

Abstract : BACKGROUND: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. RESULTS: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. CONCLUSIONS: miR-143 is as a new target for prostate cancer treatment.
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Submitted on : Thursday, November 12, 2009 - 12:31:41 PM
Last modification on : Wednesday, August 19, 2020 - 11:16:38 AM
Long-term archiving on: : Thursday, June 17, 2010 - 6:26:54 PM

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Cyrielle Clapé, Vanessa Fritz, Corinne Henriquet, Florence Apparailly, Pedro Luis Fernandez, et al.. miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice.. PLoS ONE, Public Library of Science, 2009, 4 (10), pp.e7542. ⟨10.1371/journal.pone.0007542⟩. ⟨inserm-00431462⟩

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