Skip to Main content Skip to Navigation
Journal articles

Mineralocorticoid modulation of cardiac ryanodine receptor activity is associated with downregulation of FK506-binding proteins.

Abstract : BACKGROUND: The mineralocorticoid pathway is involved in cardiac arrhythmias associated with heart failure through mechanisms that are incompletely understood. Defective regulation of the cardiac ryanodine receptor (RyR) is an important cause of the initiation of arrhythmias. Here, we examined whether the aldosterone pathway might modulate RyR function. METHODS AND RESULTS: Using the whole-cell patch clamp method, we observed an increase in the occurrence of delayed afterdepolarizations during action potential recordings in isolated adult rat ventricular myocytes exposed for 48 hours to aldosterone 100 nmol/L, in freshly isolated myocytes from transgenic mice with human mineralocorticoid receptor expression in the heart, and in wild-type littermates treated with aldosterone. Sarcoplasmic reticulum Ca(2+) load and RyR expression were not altered; however, RyR activity, visualized in situ by confocal microscopy, was increased in all cells, as evidenced by an increased occurrence and redistribution to long-lasting and broader populations of spontaneous Ca(2+) sparks. These changes were associated with downregulation of FK506-binding proteins (FKBP12 and 12.6), regulatory proteins of the RyR macromolecular complex. CONCLUSIONS: We suggest that in addition to modulation of Ca(2+) influx, overstimulation of the cardiac mineralocorticoid pathway in the heart might be a major upstream factor for aberrant Ca(2+) release during diastole, which contributes to cardiac arrhythmia in heart failure.
Complete list of metadatas

Cited literature [46 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00422024
Contributor : Ana Maria Gomez <>
Submitted on : Monday, October 5, 2009 - 3:16:44 PM
Last modification on : Wednesday, September 23, 2020 - 3:09:51 AM
Long-term archiving on: : Tuesday, June 15, 2010 - 8:31:49 PM

Files

AMGomezCIRCULATIONAHA-2008-805...
Files produced by the author(s)

Identifiers

Collections

Citation

Ana María Gómez, Angélica Rueda, Yannis Sainte-Marie, Laetitia Pereira, Spyros Zissimopoulos, et al.. Mineralocorticoid modulation of cardiac ryanodine receptor activity is associated with downregulation of FK506-binding proteins.. Circulation, American Heart Association, 2009, 119 (16), pp.2179-87. ⟨10.1161/CIRCULATIONAHA.108.805804⟩. ⟨inserm-00422024⟩

Share

Metrics

Record views

2069

Files downloads

2420