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Journal Articles Nature Methods Year : 2009

Automated unrestricted multigene recombineering for multiprotein complex production.

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Abstract

Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.
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Dates and versions

inserm-00420401 , version 1 (28-09-2009)

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Christoph Bieniossek, Yan Nie, Daniel Frey, Natacha Olieric, Christiane Schaffitzel, et al.. Automated unrestricted multigene recombineering for multiprotein complex production.. Nature Methods, 2009, 6 (6), pp.447-450. ⟨10.1038/nmeth.1326⟩. ⟨inserm-00420401⟩
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