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Automated unrestricted multigene recombineering for multiprotein complex production.

Abstract : Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.
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Contributor : Maité Peney <>
Submitted on : Monday, September 28, 2009 - 6:33:30 PM
Last modification on : Thursday, May 27, 2021 - 1:54:05 PM




Christoph Bieniossek, Yan Nie, Daniel Frey, Natacha Olieric, Christiane Schaffitzel, et al.. Automated unrestricted multigene recombineering for multiprotein complex production.. Nature Methods, Nature Publishing Group, 2009, 6 (6), pp.447-450. ⟨10.1038/nmeth.1326⟩. ⟨inserm-00420401⟩



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