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Article Dans Une Revue Cell Death and Differentiation Année : 2009

Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.

Lorenzo Galluzzi (1) , Stuart A. Aaronson (2) , John Abrams (3) , Emad S Alnemri (4) , David W. Andrews (5) , Eric H. Baehrecke (6) , Nicolas G. Bazan (7) , Mikhail V. Blagosklonny (8) , Klas Blomgren (9, 10) , Christopher Borner (11) , Dale E. Bredesen (12) , Catherine Brenner (13) , Maria Castedo (1) , John A. Cidlowski (14) , Aaron Ciechanover (15) , Gerry M. Cohen (16) , Vincenzo de Laurenzi (17) , Ruggero de Maria (18, 19) , Mohanish Deshmukh (20) , Brian D. Dynlacht (21) , Wafik S. El-Deiry (22) , Richard A. Flavell (23, 24) , Simone Fulda (25) , Carmen Garrido (26) , Pierre Golstein (27) , Marie-Lise Gougeon (28) , Douglas R. Green (29) , Hinrich Gronemeyer (30) , György Hajnóczky (31) , J. Marie Hardwick (32) , Michael O. Hengartner (33) , Hidenori Ichijo (34) , Marja Jäättelä (35) , Olivier Kepp (1) , Adi Kimchi (36) , Daniel J. Klionsky (37) , Richard A. Knight (38) , Sally Kornbluth (39) , Sharad Kumar (40) , Beth Levine (24, 41) , Stuart A. Lipton (42, 43, 44, 45) , Enrico Lugli (46) , Frank Madeo (47) , Walter Malomi (48) , Jean-Christophe W. Marine (49, 50) , Seamus J. Martin (51) , Jan Paul Medema (52, 53) , Patrick Mehlen (54, 55) , Gerry Melino (16, 56) , Ute M. Moll (57, 58, 59) , Eugenia Morselli (1) , Shigekazu Nagata (60, 61) , Donald W. Nicholson (62) , Pierluigi Nicotera (16) , Gabriel Nuñez (63) , Moshe Oren (64) , Josef Penninger (65) , Shazib Pervaiz (66, 67, 68) , Marcus E. Peter (69) , Mauro Piacentini (70, 71) , Jochen H. M. Prehn (72) , Hamsa Puthalakath (73) , Gabriel A. Rabinovich (74) , Rosario Rizzuto (75) , Cecilia M. P. Rodrigues (76) , David C. Rubinsztein (77) , Thomas Rudel (78) , Luca Scorrano (79, 80) , Hans-Uwe Simon (81) , Hermann Steller (24, 82) , Jürg Tschopp (83) , Yoshihide Tsujimoto (84) , Peter Vandenabeele (50, 85) , Ilio Vitale (1) , Karen H. Vousden (86) , Richard J. Youle (87) , Junying Yuan (88) , Boris Zhivotovsky (89) , Guido Kroemer (1)
1 U848 - Apoptose, cancer et immunité
2 Department of Oncological Sciences
3 Department of Cell Biology
4 Department of Biochemistry and Molecular Biology
5 Department of Biochemistry and Biomedical Sciences
6 Department of Cancer Biology
7 Neuroscience Center of Excellence
8 RPCI - Roswell Park Cancer Institute [Buffalo]
9 Center for Brain Repair and Rehabilitation
10 Department of Pediatric Oncology
11 ZBMZ - Institute of Molecular Medicine and Cell Research
12 Buck Institute for Age Research
13 LGBC - Laboratoire de génétique et biologie cellulaire
14 National Institutes of Environmental Health Sciences
15 Vascular and Tumor Biology Research Center
16 Toxicology Unit
17 Dipartimento di Scienze Biomediche
18 Department of Hematology, Oncology and Molecular Medicine
19 Mediterranean Institute of Oncology
20 Department of Cell and Developmental Biology
21 Department of Pathology
22 Hematology-Oncology Division
23 Section of Immunobiology
24 HHMI - Howard Hughes Medical Institute [Chevy Chase]
25 University Children's Hospital
26 LNC - Lipides - Nutrition - Cancer (U866)
27 CIML - Centre d'Immunologie de Marseille - Luminy
28 Immunité Antivirale, Biothérapie et Vaccins
29 Department of Immunology
30 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
31 Department of Pathology, Anatomy and Cell Biology
32 Department of Pharmacology and Molecular Sciences
33 Institute of Molecular Biology
34 Graduate School of Medecine
35 Danish Cancer Society
36 Department of Molecular Genetics
37 Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology and Biological Chemistry
38 Institute of Child Health [London]
39 Duke University School of Medicine
40 Centre for Cancer Biology
41 University of Texas Southwestern Medical Center [Dallas]
42 Burnham Institute for Medical Research
43 The Salk Institute for Biological Studies
44 The Scripps Research Institute [La Jolla, San Diego]
45 UC San Diego - University of California [San Diego]
46 Immunotechnology Section, Vaccine Research Center
47 Institute of Molecular Biosciences
48 Department of Therapeutic Research and Medicines Evaluation
49 Laboratory for Molecular Cancer Biology
50 Department for Molecular Biology
51 Department of Genetics
52 Center for Experimental and Molecular Medicine
53 UvA - University of Amsterdam [Amsterdam] = Universiteit van Amsterdam
54 Apoptosis, Cancer, and Development Laboratory
55 Apoptose Cancer et Développement
56 Department of Experimental Medicine and Biochemical Sciences
57 Department of Pathology
58 Department of Molecular Oncology
59 Faculty of Medicine
60 Fujitsu Laboratories LTD.
61 Department of Medical Chemistry
62 Merck & Co. Inc
63 University of Michigan Medical School [Ann Arbor]
64 Department of Molecular Cell Biology [Rehovot]
65 Institute of Molecular Biotechnology
66 Department of Physiology
67 SMART - Singapore-MIT Alliance for Research and Technology
68 Duke-NUS Medical School [Singapore]
69 Ben May Department for Cancer Research
70 Laboratory of Cell Biology
71 Department of Biology
72 Department of Physiology and Medical Physics
73 Department of Biochemistry
74 Laboratorie de Inmunopatologia
75 Department of Biomedical Sciences
76 iMed.UL
77 CIMR - Cambridge Institute for Medical Research
78 Biocenter
79 Department of Cell Physiology and Metabolism
80 Dulbecco-Telethon Institute
81 Department of Pharmacology and Molecular Sciences
82 Laboratory of Apoptosis and Cancer Biology
83 Department of Biochemistry [Lausanne]
84 Department of Medical Genetics
85 Department for Molecular Biomedical Research
86 The Beatson Institute for Cancer Research
87 Biochemistry Section, Surgical Neurology Branch
88 Department of cell biology
89 Division of Toxicology
Lorenzo Galluzzi
Gerry M. Cohen
  • Fonction : Auteur
Cecilia M. P. Rodrigues
  • Fonction : Auteur
Thomas Rudel
  • Fonction : Auteur
Guido Kroemer

Résumé

Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.

Dates et versions

inserm-00420382 , version 1 (28-09-2009)

Identifiants

Citer

Lorenzo Galluzzi, Stuart A. Aaronson, John Abrams, Emad S Alnemri, David W. Andrews, et al.. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.. Cell Death and Differentiation, 2009, 16 (8), pp.1093-107. ⟨10.1038/cdd.2009.44⟩. ⟨inserm-00420382⟩
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