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Journal articles
Correlation between invasiveness of colorectal tumor cells and adhesive potential under flow.
Abstract : BACKGROUND: Tumor cell adhesiveness is involved in metastatic dissemination, and adhesive behavior may be different under static and dynamic conditions. MATERIALS AND METHODS: Patients undergoing primary colorectal cancer excision were tested for: i) serum concentration of sE-selectin, sICAM-1 and sVCAM-1, ii) expression of CD18, CD29d and E-cadherin on tumor cells and iii) efficiency of tumor cell adhesion to ECV304 monolayers under flow and resistance to detachment by shear. RESULTS: Twenty out of 31 patients were free of detectable relapse 12 months later. Relapsing and non-relapsing patients had similar levels of soluble adhesion molecules. E-cadherin was detected on tumor cells from three non-relapsing patients, but no relapsing one. Unexpectedly, significant CD18 labeling was found on two relapsing patients and one non-relapsing patient. Cells from relapsing patients displayed significantly increased (p < 0.05 two-sided, p < 0.025 one-sided) capacity to adhere to test monolayers under flow. CONCLUSION: Cancer invasion is related to tumor cell adhesiveness, and the flow chamber provides a practical way of measuring adhesive parameters with a potential value for relapse prediction.
Cited literature [32 references]
https://www.hal.inserm.fr/inserm-00420375
Contributor : Pierre Bongrand <>
Submitted on : Monday, September 28, 2009 - 5:39:45 PM
Last modification on : Wednesday, August 19, 2020 - 12:08:06 PM
Long-term archiving on: : Saturday, November 26, 2016 - 12:12:51 PM
Contributor : Pierre Bongrand <>
Submitted on : Monday, September 28, 2009 - 5:39:45 PM
Last modification on : Wednesday, August 19, 2020 - 12:08:06 PM
Long-term archiving on: : Saturday, November 26, 2016 - 12:12:51 PM