Abstract : We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an intercalating fluorochrome, allowing fast and accurate "digital" quantification of the template DNA based on the Poisson distribution of DNA molecules in droplets. The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system.
Type de document :
Article dans une revue
Analytical Chemistry, American Chemical Society, 2009, 81 (12), pp.4813-21. 〈10.1021/ac900403z〉
Liste complète des métadonnées
http://www.hal.inserm.fr/inserm-00420178
Contributeur : Maité Peney
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Soumis le : lundi 28 septembre 2009 - 13:29:20
Dernière modification le : jeudi 11 janvier 2018 - 06:17:42
Linas Mazutis, Ali Fallah Araghi, Oliver Miller, Jean-Christophe Baret, Lucas Frenz, et al.. Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis.. Analytical Chemistry, American Chemical Society, 2009, 81 (12), pp.4813-21. 〈10.1021/ac900403z〉. 〈inserm-00420178〉
