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Journal articles
Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis.
Linas Mazutis
1
Ali Fallah Araghi
1
Oliver Miller
1
Jean-Christophe Baret
1
Lucas Frenz
1
Agnes Janoshazi
1, 2
Valérie Taly
1
Benjamin Miller
3
J Brian Hutchison
3
Darren Link
3
Andrew Griffiths
1, 2
Michael Ryckelynck
1, 2
Linas Mazutis 1
Author
Ali Fallah Araghi 1
Author
Oliver J. Miller 1
Author
Jean-Christophe Baret 1
Author IdHAL : jean-christophe-baret ResearcherId : http://www.researcherid.com/rid/E-1912-2016 ORCID : https://orcid.org/0000-0003-2048-8317
Lucas Frenz 1
Author
Agnes Janoshazi 1, 2
Author
Valérie Taly 1
Author IdHAL : valerie-taly
Benjamin J. Miller 3
Author
J Brian Hutchison 3
Author
Darren R. Link 3
Author
Andrew D. Griffiths 1, 2
Author
Michael Ryckelynck 1, 2
Author
1
ISIS - Institut de Science et d'ingénierie supramoléculaires (ISIS 8, Allée Gaspard Monge - BP 70028 67083 STRASBOURG CEDEX - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7006 (France)
- Université Louis Pasteur - Strasbourg I (France)
2
IGBMC - Institut de génétique et biologie moléculaire et cellulaire (I.G.B.M.C, Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 ILLKIRCH CEDEX - France)
- Université Louis Pasteur - Strasbourg I (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U596 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7104 (France)
3
RainDance Technologies, Inc. (44 Hartwell Avenue, Lexington, Massachusetts 02421 - United States)
- RainDance Technologies, Inc. (France)
Abstract : We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an intercalating fluorochrome, allowing fast and accurate "digital" quantification of the template DNA based on the Poisson distribution of DNA molecules in droplets. The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system.
Document type :
Journal articles
Complete list of metadata
https://www.hal.inserm.fr/inserm-00420178
Contributor : Maité Peney <>
Submitted on : Monday, September 28, 2009 - 1:29:20 PM
Last modification on : Tuesday, September 29, 2020 - 4:58:02 PM
Contributor : Maité Peney <>
Submitted on : Monday, September 28, 2009 - 1:29:20 PM
Last modification on : Tuesday, September 29, 2020 - 4:58:02 PM
Identifiers
- HAL Id : inserm-00420178, version 1
- DOI : 10.1021/ac900403z
- PUBMED : 19518143
Citation
Linas Mazutis, Ali Fallah Araghi, Oliver Miller, Jean-Christophe Baret, Lucas Frenz, et al.. Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis.. Analytical Chemistry, American Chemical Society, 2009, 81 (12), pp.4813-21. ⟨10.1021/ac900403z⟩. ⟨inserm-00420178⟩
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