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Chronic TNFalpha and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis.

Abstract : We examined the effects of chronic TNFalpha and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNFalpha decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNFalpha and Db-cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis.
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https://www.hal.inserm.fr/inserm-00419424
Contributor : Marie Francoise Simon <>
Submitted on : Wednesday, September 23, 2009 - 4:00:58 PM
Last modification on : Friday, January 10, 2020 - 9:09:41 PM

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Véronic Bézaire, Aline Mairal, Rodica Anesia, Corinne Lefort, Dominique Langin. Chronic TNFalpha and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis.. FEBS Letters, Wiley, 2009, 583 (18), pp.3045-9. ⟨10.1016/j.febslet.2009.08.019⟩. ⟨inserm-00419424⟩

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