Intrabody expression in eukaryotic cells. - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2009

Intrabody expression in eukaryotic cells.

(1, 2) , (1, 2)
1
2

Abstract

We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
Fichier principal
Vignette du fichier
inserm-00418821_edited.pdf (159.31 Ko) Télécharger le fichier
Vignette du fichier
intrabodies.pdf (256.82 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

inserm-00418821 , version 1 (21-09-2009)

Identifiers

Cite

Laurence Guglielmi, Pierre Martineau. Intrabody expression in eukaryotic cells.. Methods in Molecular Biology, 2009, 562, pp.195-203. ⟨10.1007/978-1-60327-302-2_15⟩. ⟨inserm-00418821⟩
129 View
564 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More