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Journal articles
Intrabody expression in eukaryotic cells.
Abstract : We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
https://www.hal.inserm.fr/inserm-00418821
Contributor : Yves Le Ster Connect in order to contact the contributor
Submitted on : Monday, September 21, 2009 - 4:59:41 PM
Last modification on : Tuesday, March 15, 2022 - 9:35:57 AM
Long-term archiving on: : Tuesday, June 15, 2010 - 8:51:17 PM
Contributor : Yves Le Ster Connect in order to contact the contributor
Submitted on : Monday, September 21, 2009 - 4:59:41 PM
Last modification on : Tuesday, March 15, 2022 - 9:35:57 AM
Long-term archiving on: : Tuesday, June 15, 2010 - 8:51:17 PM
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