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Intrabody expression in eukaryotic cells.

Abstract : We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
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https://www.hal.inserm.fr/inserm-00418821
Contributor : Yves Le Ster <>
Submitted on : Monday, September 21, 2009 - 4:59:41 PM
Last modification on : Wednesday, October 3, 2018 - 4:02:06 PM
Long-term archiving on: : Tuesday, June 15, 2010 - 8:51:17 PM

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Laurence Guglielmi, Pierre Martineau. Intrabody expression in eukaryotic cells.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2009, 562, pp.195-203. ⟨10.1007/978-1-60327-302-2_15⟩. ⟨inserm-00418821⟩

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