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Expression of Single-Chain Fv Fragments in E. coli Cytoplasm.

Abstract : The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.
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https://www.hal.inserm.fr/inserm-00418812
Contributor : Yves Le Ster <>
Submitted on : Monday, September 21, 2009 - 4:40:16 PM
Last modification on : Wednesday, October 3, 2018 - 4:02:06 PM
Long-term archiving on: : Saturday, November 26, 2016 - 12:31:05 PM

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Laurence Guglielmi, Pierre Martineau. Expression of Single-Chain Fv Fragments in E. coli Cytoplasm.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2009, 562, pp.215-24. ⟨10.1007/978-1-60327-302-2_17⟩. ⟨inserm-00418812⟩

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