Skip to Main content Skip to Navigation
Journal articles

Expression of Single-Chain Fv Fragments in E. coli Cytoplasm.

Abstract : The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.
Document type :
Journal articles
Complete list of metadata

Cited literature [13 references]  Display  Hide  Download
Contributor : Yves Le Ster Connect in order to contact the contributor
Submitted on : Monday, September 21, 2009 - 4:40:16 PM
Last modification on : Tuesday, March 15, 2022 - 9:35:58 AM
Long-term archiving on: : Saturday, November 26, 2016 - 12:31:05 PM


Files produced by the author(s)



Laurence Guglielmi, Pierre Martineau. Expression of Single-Chain Fv Fragments in E. coli Cytoplasm.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2009, 562, pp.215-24. ⟨10.1007/978-1-60327-302-2_17⟩. ⟨inserm-00418812⟩



Record views


Files downloads