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Muco-ciliary differentiation of nasal epithelial cells is decreased after wound healing in vitro.: Muco-ciliary differentiation & wound healing

Diane Lazard 1, * Anne Moore 2 Vincent Hupertan 3 Clémence Martin 4 Virginie Escabasse 5 Patrick Dreyfus 6 Pierre-Régis Burgel 7, 8 Serge Amselem 2 Estelle Escudier 2, * André Coste 5
* Corresponding author
1 INSERM U955, équipe 13
IMRB - Institut Mondor de Recherche Biomédicale
4 INSERM U955, équipe 13
Service de pneumologie [CHU Cochin], IMRB - Institut Mondor de Recherche Biomédicale, EA 2511 - Mucoviscidose et bronchopathies chroniques : biopathologie et phénotypes cliniques
5 INSERM U955, équipe 13
Service d'ORL et de Chirurgie Cervico-Faciale, Service d'ORL et de chirurgie cervico-faciale, IMRB - Institut Mondor de Recherche Biomédicale
Abstract : BACKGROUND: Epithelial damage and modifications of cell differentiation are frequent in airway diseases with chronic inflammation, in which transforming growth factor-beta1 (TGF-beta1) plays an important role. The aim of this study was to evaluate the differentiation of human nasal epithelial cells (HNEC) after wound healing and the potential effects of TGF-beta1. METHODS: Basal, mucus, and ciliated cells were characterized by cytokeratin-14, MUC5AC, and betaIV tubulin immunodetection, respectively. Their expression was evaluated in situ in nasal polyps and in an in vitro model of wound healing in primary cultures of HNEC after wound closure, under basal conditions and after TGF-beta1 supplementation. Using RT-PCR, the effects of TGF-beta1 on MUC5AC and DNAI1 genes, specifically transcribed in mucus and ciliated cells, were evaluated. RESULTS: In situ, high TGF-beta1 expression was associated with low MUC5AC and betaIV tubulin expression. In vitro, under basal conditions, MUC5AC expression remained stable, cytokeratin-14 expression was strong and decreased with time, while betaIV tubulin expression increased. Transforming growth factor-beta1 supplementation downregulated MUC5AC and betaIV tubulin expression as well as MUC5AC and DNAI1 transcripts. CONCLUSION: After a wound, differentiation into mucus and ciliated cells was possible and partially inhibited in vitro by TGF-beta1, a cytokine that may be involved in epithelial remodeling observed in chronic airway diseases.
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https://www.hal.inserm.fr/inserm-00414660
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Submitted on : Tuesday, February 23, 2010 - 2:02:41 PM
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Diane Lazard, Anne Moore, Vincent Hupertan, Clémence Martin, Virginie Escabasse, et al.. Muco-ciliary differentiation of nasal epithelial cells is decreased after wound healing in vitro.: Muco-ciliary differentiation & wound healing. Allergy, Wiley, 2009, 64 (8), pp.1136-43. ⟨10.1111/j.1398-9995.2009.02003.x⟩. ⟨inserm-00414660⟩

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