Skip to Main content Skip to Navigation
Journal articles

Knocking out the regulatory beta subunit of protein kinase CK2 in mice: gene dosage effects in ES cells and embryos.

Abstract : Knocking out the regulatory beta subunit of protein kinase CK2 in mice leads to early embryonic lethality. Heterozygous CK2beta (CK2beta+/-) knockout mice do not show an obvious phenotype. However, the number of heterozygous offsprings from CK2B+/- inter-crossings is lower than expected, meaning that some heterozygous embryos do not survive. Interestingly, CK2beta+/- ES (Embryonic Stem) cells express a considerably lower level of CK2beta than wild-type ES cells, whereas the level of CK2beta in organs from heterozygous adult mice does not significantly differ from those of wild-type mice. The data suggest a compensatory mechanism that adjusts CK2beta levels during development in the majority of, but not in all, cases (Mol Cell Biol 23: 908-915, 2003). In order to find an explanation for the gene dosage effect observed for heterozygous offsprings, we analysed embryos at mid-gestation (E10.5) as well as wild-type and CK2beta+/- ES cells for differences in growth rate and response to different stress agents. Analysis of E10.5 embryos generated from heterozygous matings revealed about 20% of smaller retarded CK2beta+/- embryos. No correlation between CK2beta levels in normal looking and retarded CK2beta+/- embryos were found. However, a different post-translational form of CK2beta protein has been detected in these retarded embryos. Cellular parameters such as growth rate and G1-, G2-checkpoints in ES cells were identical in both wild-type and CK2beta+/- cells. When ES cells were injected to induce differentiated teratocarcinoma in syngenic mice, the size of the tumours correlated with the level of CK2beta.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00413204
Contributor : Claude Cochet <>
Submitted on : Thursday, September 3, 2009 - 2:38:53 PM
Last modification on : Friday, August 23, 2019 - 3:20:03 PM

Identifiers

  • HAL Id : inserm-00413204, version 1
  • PUBMED : 16335526

Collections

CEA

Citation

Olivier Blond, Hans Jensen, Thierry Buchou, Claude Cochet, Olaf-Georg Issinger, et al.. Knocking out the regulatory beta subunit of protein kinase CK2 in mice: gene dosage effects in ES cells and embryos.. Molecular and Cellular Biochemistry, Springer Verlag, 2005, 274 (1-2), pp.31-7. ⟨inserm-00413204⟩

Share

Metrics

Record views

154