The VEGF IRESes are differentially susceptible to translation inhibition by miR-16.

Zeïneb Karaa 1 Jason Iacovoni 1 Amandine Bastide 1 Eric Lacazette 1 Christian Touriol 1 Hervé Prats 1, *
* Auteur correspondant
1 I2MR - Institut de médecine moléculaire de Rangueil
Abstract : Experiments with EMCV (Encephalomyocarditis virus) internal ribosome entry sites (IRESes) have shown that microRNAs (miRs) are unable to inhibit IRES driven translation. However, it is accepted that miRs can inhibit translation through multiple mechanisms, only some of which require interaction with the 5' cap structure. In this report, we first validate the targeting of miR-16 to a predicted binding site in the VEGF 3'UTR. We developed a series of experiments to ascertain whether or not miR-16 can inhibit translation of transcripts driven by either of the VEGF IRESes. Our results indicate that cellular IRESes can be classified as both sensitive and insensitive to miR control. While VEGF IRES-A activity was not altered by miR-16 targeting to the 3'UTR, IRES-B was susceptible to miR-16 inhibition. Taken together with previous results that show that IRES-B selectively translates the CUG initiated VEGF-121 isoform, we can conclude that the existence of two differentially susceptible IRESes in the VEGF 5'UTR leads to even more complex regulatory control of VEGF isoform production. This study demonstrates for the first time the inhibition of cellular IRES driven translation by a miR.
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RNA, Cold Spring Harbor Laboratory Press, 2009, 15 (2), pp.249-54. 〈10.1261/rna.1301109〉
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http://www.hal.inserm.fr/inserm-00410605
Contributeur : Marie Francoise Simon <>
Soumis le : vendredi 21 août 2009 - 15:38:46
Dernière modification le : jeudi 13 septembre 2018 - 10:26:05

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Zeïneb Karaa, Jason Iacovoni, Amandine Bastide, Eric Lacazette, Christian Touriol, et al.. The VEGF IRESes are differentially susceptible to translation inhibition by miR-16.. RNA, Cold Spring Harbor Laboratory Press, 2009, 15 (2), pp.249-54. 〈10.1261/rna.1301109〉. 〈inserm-00410605〉

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