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Proteomic study of the impact of the JAK2-V617F mutation on the phenotype of essential thrombocythemia.

Abstract : OBJECTIVE: Previous studies have suggested that two subtypes of essential thrombocythemia (ET) could be separated on the basis of their JAK2 status (V617F-positive or V617F-negative), with a continuum between V617F-positive ET and polycythemia vera (PV). Nevertheless, increasingly contradictory data on the impact of JAK2-V617F (presence and load) on ET phenotype highlight the need for further investigations. MATERIALS AND METHODS: We investigated the influence of JAK2-V617F on ET phenotype using mass spectrometry-based analysis of serum protein profiles of ET patients, comparatively with PV patients. RESULTS: V617F-positive ET and PV displayed significant differences in their serum protein profiles. Furthermore, JAK2-V617F presence did not impact significantly the serum proteome of ET patients: we observed very few differences in serum protein profiles of V617F-positive and -negative ET. Reciprocally, clustering of ET patients on the basis of their serum protein profiles did not correlate with JAK2-V617F presence. Finally, the JAK2-V617F load did not influence serum apolipoprotein A-1 levels in ET, a previously validated marker of JAK2-V617F allele burden in PV. CONCLUSION: Serum proteome of ET patients was not influenced by the presence of JAK2-V617F or by high V617F allelic ratio (up to 50%) suggesting that ET phenotype is, at best, only partially influenced by the JAK2-V617F mutation.
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Contributor : Jean-Paul Issartel Connect in order to contact the contributor
Submitted on : Friday, August 21, 2009 - 10:34:43 AM
Last modification on : Wednesday, July 21, 2021 - 10:56:02 AM

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Pascal Mossuz, Marie Arlotto, Sylvie Hermouet, Ali Bouamrani, Eric Lippert, et al.. Proteomic study of the impact of the JAK2-V617F mutation on the phenotype of essential thrombocythemia.. Experimental Hematology, Elsevier, 2008, 36 (12), pp.1642-7. ⟨10.1016/j.exphem.2008.07.013⟩. ⟨inserm-00410498⟩



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