In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Biomedical Optics Year : 2008

In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B.

Abstract

Staining and imaging glial cells in vivo while observing the microvasculature could help understand brain physiology, namely neuronal-glial-vascular communication. Two-photon excitation microscopy provides a means to monitor these interactions at the cellular level in living animals, but the cells of interest must be fluorescent. Injecting dyes intravenously is a rapid and quasi noninvasive method to stain cells in the brain. It necessitates that the dye is soluble in the blood plasma and crosses the blood brain barrier (BBB). We demonstrate here, using two-photon imaging, that sulforhodamine B (SRB) crosses the BBB and stains in vivo, specifically mouse astrocytes. This is confirmed by experiments on primary neurons and astrocytes cultures showing the preferential SRB staining of the latter. SRB is rapidly eliminated from the blood, which allows repeated injections in longitudinal studies.
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Dates and versions

inserm-00410456 , version 1 (20-08-2009)

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Pascale Vérant, Clément Ricard, Raphaël Serduc, Jean-Claude Vial, Boudewijn van Der Sanden. In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B.. Journal of Biomedical Optics, 2008, 13 (6), pp.064028. ⟨10.1117/1.3041163⟩. ⟨inserm-00410456⟩
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