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Fluorescent Pluronic nanodots for in vivo two-photon imaging.

Abstract : We report the synthesis of new nanosized fluorescent probes based on bio-compatible polyethylene-polypropylene glycol (Pluronic) materials. In aqueous solution, mini-emulsification of Pluronic with a high fluorescent di-stryl benzene-modified derivative, exhibiting a two-photon absorption cross section as high as 2500 Goeppert-Mayer units at 800 nm, leads to nanoparticles exhibiting a hydrodynamic radius below 100 nm. We have demonstrated that these new probes with luminescence located in the spectral region of interest for bio-imaging (the yellow part of the visible spectrum) allow deep (500 microm) bio-imaging of the mice brain vasculature. The dose injected during our experiments is ten times lower when compared to the classical commercial rhodamine-B isothicyanate-Dextran system but gives similar results to homogeneous blood plasma staining. The mean fluorescent signal intensity stayed constant during more than 1 h.
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Contributor : Raphael Serduc <>
Submitted on : Thursday, August 20, 2009 - 4:03:01 PM
Last modification on : Wednesday, May 5, 2021 - 9:38:03 AM

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Mathieu Maurin, Laeticia Vurth, Jean-Claude Vial, Patrice Baldeck, Seth Marder, et al.. Fluorescent Pluronic nanodots for in vivo two-photon imaging.. Nanotechnology, Institute of Physics, 2009, 20 (23), pp.235102. ⟨10.1088/0957-4484/20/23/235102⟩. ⟨inserm-00410443⟩



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