Adsorption and release of BMP-2 on nanocrystalline apatite-coated and uncoated hydroxyapatite/beta-tricalcium phosphate porous ceramics.

Hélène Autefage 1, 2, * Fabienne Briand-Mésange 3 Sophie Cazalbou 4 Christophe Drouet 2 Daniel Fourmy 5 Stéphane Gonçalvès 4 Jean-Pierre Salles 3 Christèle Combes 2 Pascal Swider 1 Christian Rey 2
* Auteur correspondant
1 Laboratoire de biomécanique de Toulouse (EA 3697)
2 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux
3 Centre de Physiopathologie Toulouse Purpan (ex IFR 30 et IFR 150)
4 Teknimed
5 I2MR - Institut de médecine moléculaire de Rangueil
Abstract : The association of bone morphogenetic proteins (BMPs) with calcium phosphate bioceramics is known to confer them osteoinductive properties. The aim of this study was to evaluate the surface properties, especially regarding recombinant human BMP-2 (rhBMP-2) adsorption and release, of commercial sintered biphasic calcium phosphate ceramics after coating with biomimetic nanocrystalline apatite. The raw and coated ceramics exhibited similar macroporous structures but different nanometer-sized pores contents. Both types of ceramics showed Langmuir-type adsorption isotherms of rhBMP-2. The coating noticeably increased the rate of adsorption and the total amount of growth factor taken up, but the maximum coverage per surface area unit as well as the affinity constant appeared lower for coated ceramics compared with raw ceramic surfaces. The limited advantage gained by coating the ceramics can be assigned to a lower accessibility of the surface adsorption sites compared with the raw ceramics. The quantity of rhBMP-2 spontaneously released in cell culture medium during the first weeks was lower for coated samples than for uncoated ceramics and represented a minor fraction of the total adsorbed amount. In conclusion, the nanocrystalline apatite coating was found to favor the adsorption of rhBMP-2 while providing a mean to fine tune the release of the growth factor. (c) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009.
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Article dans une revue
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Wiley, 2009, epub ahead of print. 〈10.1002/jbm.b.31447〉
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Contributeur : Marie Francoise Simon <>
Soumis le : jeudi 20 août 2009 - 15:25:40
Dernière modification le : vendredi 14 septembre 2018 - 08:42:04

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Hélène Autefage, Fabienne Briand-Mésange, Sophie Cazalbou, Christophe Drouet, Daniel Fourmy, et al.. Adsorption and release of BMP-2 on nanocrystalline apatite-coated and uncoated hydroxyapatite/beta-tricalcium phosphate porous ceramics.. Journal of Biomedical Materials Research Part B: Applied Biomaterials, Wiley, 2009, epub ahead of print. 〈10.1002/jbm.b.31447〉. 〈inserm-00410432〉

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