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Two structurally identical mannose-specific jacalin-related lectins display different effects on human T lymphocyte activation and cell death.

Abstract : Plant lectins displaying similar single sugar-binding specificity and identical molecular structure might present various biological effects. To explore this possibility, the effects on human lymphocytes of two mannose-specific and structurally closely related lectins, Morniga M from Morus nigra and artocarpin from Artocarpus integrifolia were investigated. In silico analysis revealed that Morniga M presents a more largely open carbohydrate-binding cavity than artocarpin, probably allowing interactions with a broader spectrum of carbohydrate moieties. In vitro, Morniga M interacted strongly with the lymphocyte surface and was uptaken quickly by cells. Morniga M and artocarpin triggered the proliferation and activation of human T and NK lymphocytes. A minority of B lymphocytes was activated in artocarpin-treated culture, whereas Morniga M favored the emergence of CD4+ CD8+ T lymphocytes. Moreover, cell death occurred in activated PBMC, activated T lymphocytes, and Jurkat T leukemia cells incubated with Morniga M only. The biological effects of both lectins were dependent on carbohydrate recognition. The Morniga M-induced cell death resulted, at least in part, from caspase-dependent apoptosis and FADD-dependent receptor-mediated cell death. Finally, Morniga M, but not artocarpin, triggered AICD of T lymphocytes. In conclusion, both lectins trigger lymphocyte activation, but only Morniga M induces cell death. In spite of similar in vitro mannose-binding specificities and virtually identical structure, only Morniga M probably interacts with carbohydrate moieties bound to molecules able to induce cell death. The present data suggest that subtle alterations in N-glycans can distinguish activation and cell death molecules at the lymphocyte surface.
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https://www.hal.inserm.fr/inserm-00409940
Contributor : Marie Francoise Simon <>
Submitted on : Friday, August 14, 2009 - 2:26:13 PM
Last modification on : Friday, January 10, 2020 - 9:09:41 PM

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Hervé Benoist, Raphaël Culerrier, Guillaume Poiroux, Bruno Ségui, Alain Jauneau, et al.. Two structurally identical mannose-specific jacalin-related lectins display different effects on human T lymphocyte activation and cell death.. Journal of Leukocyte Biology, Society for Leukocyte Biology, 2009, 86 (1), pp.103-14. ⟨10.1189/jlb.0708434⟩. ⟨inserm-00409940⟩

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