Skip to Main content Skip to Navigation
Journal articles

Longitudinal analysis of gene expression in porcine skeletal muscle after post-injection local injury.

Abstract : PURPOSE: The purpose of this study is to describe the time course of gene expression in a skeletal muscle local injury induced by an intramuscular (IM) injection, and to compare the dynamics of gene expression with pathological events. MATERIALS AND METHODS: Ten piglets received 4 IM injections of propylene glycol in the longissimus dorsi muscles 6 h, 2, 7, and 21 days before euthanasia, where control and injected muscle sites were sampled for RNA isolation and microscopic examination. The hybridization of nylon cDNA microarrays was carried out with radioactive probes obtained from the muscle RNA. RESULTS: 153 genes were found under- or over-expressed at least once among the investigated time-conditions. The eight most discriminant genes were also identified: Two genes (GTP-binding protein RAD and Ankyrin repeat domain protein) were over-expressed at 6 h and six genes between 2 and 21 days (Osteonectin, Fibronectin, Matrix metalloproteinase-2, Collagen alpha 1(I) chain, Collagen alpha 2(I) chain, and Thymosin beta-4). Necrosis, inflammation and regeneration were observed through both the dynamics of gene expression profiles and through the microscopic examinations. CONCLUSION: Our data demonstrate that several pathways are involved in post-injection muscle injury, and that necrosis, inflammation and regeneration are not sequential but occur in parallel.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00409866
Contributor : Marie Francoise Simon <>
Submitted on : Thursday, August 13, 2009 - 3:10:38 PM
Last modification on : Wednesday, October 14, 2020 - 3:43:49 AM

Links full text

Identifiers

Collections

Citation

Pierre Ferré, Laurence Liaubet, Didier Concordet, Magali Sancristobal, Emmanuelle Uro-Coste, et al.. Longitudinal analysis of gene expression in porcine skeletal muscle after post-injection local injury.. Pharmaceutical Research, American Association of Pharmaceutical Scientists, 2007, 24 (8), pp.1480-9. ⟨10.1007/s11095-007-9266-8⟩. ⟨inserm-00409866⟩

Share

Metrics

Record views

443