Brain glucagon-like peptide-1 regulates arterial blood flow, heart rate, and insulin sensitivity.

Cendrine Cabou 1, 2, 3 Gérard Campistron 1, 2, 3 Nicolas Marsollier 4 Corinne Leloup 2, 5 Celine Cruciani-Guglielmacci 4 Luc Pénicaud 2, 5 Daniel Drucker 6 Christophe Magnan 4 Rémy Burcelin 1, 2, *
* Auteur correspondant
1 I2MR - Institut de médecine moléculaire de Rangueil
2 IFR 31 - IFR 31 Louis Bugnard
3 Faculté des Sciences Pharmaceutiques
4 LPPN - Laboratoire de physiopathologie de la nutrition
5 Métabolisme, plasticité et mitochondrie
6 Banting and Best Diabetes Centre
Abstract : OBJECTIVE: To ascertain the importance and mechanisms underlying the role of brain glucagon-like peptide (GLP)-1 in the control of metabolic and cardiovascular function. GLP-1 is a gut hormone secreted in response to oral glucose absorption that regulates glucose metabolism and cardiovascular function. GLP-1 is also produced in the brain, where its contribution to central regulation of metabolic and cardiovascular homeostasis remains incompletely understood. RESEARCH DESIGN AND METHODS: Awake free-moving mice were infused with the GLP-1 receptor agonist exendin-4 (Ex4) into the lateral ventricle of the brain in the basal state or during hyperinsulinemic eu-/hyperglycemic clamps. Arterial femoral blood flow, whole-body insulin-stimulated glucose utilization, and heart rates were continuously recorded. RESULTS: A continuous 3-h brain infusion of Ex4 decreased femoral arterial blood flow and whole-body glucose utilization in the awake free-moving mouse clamped in a hyperinsulinemic-hyperglycemic condition, only demonstrating that this effect was strictly glucose dependent. However, the heart rate remained unchanged. The metabolic and vascular effects of Ex4 were markedly attenuated by central infusion of the GLP-1 receptor (GLP-1R) antagonist exendin-9 (Ex9) and totally abolished in GLP-1 receptor knockout mice. A correlation was observed between the metabolic rate and the vascular flow in control and Ex4-infused mice, which disappeared in Ex9 and GLP-1R knockout mice. Moreover, hypothalamic nitric oxide synthase activity and the concentration of reactive oxygen species (ROS) were also reduced in a GLP-1R-dependent manner, whereas the glutathione antioxidant capacity was increased. Central GLP-1 activated vagus nerve activity, and complementation with ROS donor dose-dependently reversed the effect of brain GLP-1 signaling on peripheral blood flow. CONCLUSIONS: Our data demonstrate that central GLP-1 signaling is an essential component of circuits integrating cardiovascular and metabolic responses to hyperglycemia.
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Article dans une revue
Diabetes, American Diabetes Association, 2008, 57 (10), pp.2577-87. 〈10.2337/db08-0121〉
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http://www.hal.inserm.fr/inserm-00408890
Contributeur : Marie Francoise Simon <>
Soumis le : mardi 4 août 2009 - 10:14:40
Dernière modification le : jeudi 13 septembre 2018 - 10:30:07

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Cendrine Cabou, Gérard Campistron, Nicolas Marsollier, Corinne Leloup, Celine Cruciani-Guglielmacci, et al.. Brain glucagon-like peptide-1 regulates arterial blood flow, heart rate, and insulin sensitivity.. Diabetes, American Diabetes Association, 2008, 57 (10), pp.2577-87. 〈10.2337/db08-0121〉. 〈inserm-00408890〉

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