Skip to Main content Skip to Navigation
Journal articles

Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81.

Abstract : Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV pseudotype particles, and modulate HCV infection. However, the functional role of SR-BI for productive HCV infection remains unclear. In this study, we investigated the role of SR-BI as an entry factor for infection of human hepatoma cells using cell culture-derived HCV (HCVcc). Anti-SR-BI antibodies directed against epitopes of the human SR-BI extracellular loop specifically inhibited HCVcc infection in a dose-dependent manner. Down-regulation of SR-BI expression by SR-BI-specific short interfering RNAs (siRNAs) markedly reduced the susceptibility of human hepatoma cells to HCVcc infection. Kinetic studies demonstrated that SR-BI acts predominately after binding of HCV at an entry step occurring at a similar time point as CD81-HCV interaction. Although the addition of high-density lipoprotein (HDL) enhanced the efficiency of HCVcc infection, anti-SR-BI antibodies and SR-BI-specific siRNA efficiently inhibited HCV infection independent of lipoprotein. Conclusion: Our data suggest that SR-BI (i) represents a key host factor for HCV entry, (ii) is implicated in the same HCV entry pathway as CD81, and (iii) targets an entry step closely linked to HCV-CD81 interaction.
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00395706
Contributor : Thomas F. Baumert <>
Submitted on : Tuesday, November 3, 2009 - 10:22:49 AM
Last modification on : Thursday, November 26, 2020 - 3:56:04 PM
Long-term archiving on: : Thursday, June 10, 2010 - 10:34:52 PM

Files

Zeisel_et_al_Fig_6_HEP-07-0476...
Files produced by the author(s)

Identifiers

Collections

Citation

Mirjam Zeisel, George Koutsoudakis, Eva Schnober, Anita Haberstroh, Hubert Blum, et al.. Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81.. Hepatology, Wiley-Blackwell, 2007, 46 (6), pp.1722-31. ⟨10.1002/hep.21994⟩. ⟨inserm-00395706⟩

Share

Metrics

Record views

2019

Files downloads

1635