Comparative analysis of the bioenergetics of adult cardiomyocytes and nonbeating HL-1 cells: respiratory chain activities, glycolytic enzyme profiles, and metabolic fluxes.

Claire Monge 1 Nathalie Beraud 1 Kersti Tepp 2 Sophie Pelloux 3 Siham Chahboun 4 Tuuli Käämbre 2 Lumme Kadaja 5 Mart Roosimaa 5 Andres Piirsoo 5 Yves Tourneur 6 Andrei Kuznetsov 7 Valdur Saks 1 Enn Seppet 5
1 LBFA - Laboratoire de bioénergétique fondamentale et appliquée
2 Laboratory of Bioenergetics
3 Cardioprotection
4 Laboratoire de Biochimie et de Toxicologie
5 Department of Pathophysiology
6 Cardioprotection
7 Daniel Swarovski Research Laboratory
Abstract : Comparative analysis of the bioenergetic parameters of adult rat cardiomyocytes (CM) and HL-1 cells with very different structure but similar cardiac phenotype was carried out with the aim of revealing the importance of the cell structure for regulation of its energy fluxes. Confocal microscopic analysis showed very different mitochondrial arrangement in these cells. The cytochrome content per milligram of cell protein was decreased in HL-1 cells by a factor of 7 compared with CM. In parallel, the respiratory chain complex activities were decreased by 4-8 times in the HL-1 cells. On the contrary, the activities of glycolytic enzymes, hexokinase (HK), and pyruvate kinase (PK) were increased in HL-1 cells, and these cells effectively transformed glucose into lactate. At the same time, the creatine kinase (CK) activity was significantly decreased in HL-1 cells. In conclusion, the results of this study comply with the assumption that in contrast to CM in which oxidative phosphorylation is a predominant provider of ATP and the CK system is a main carrier of energy from mitochondria to ATPases, in HL-1 cells the energy metabolism is based mostly on the glycolytic reactions coupled to oxidative phosphorylation through HK.
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Article dans une revue
Canadian Journal of Physiology and Pharmacology, NRC Research Press, 2009, 87 (4), pp.318-26. 〈10.1139/y09-018〉
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http://www.hal.inserm.fr/inserm-00391388
Contributeur : Sarah Hamant <>
Soumis le : mercredi 3 juin 2009 - 18:21:50
Dernière modification le : mercredi 7 novembre 2018 - 16:07:41

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UGA | UNIV-LYON1 | LBFA

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Claire Monge, Nathalie Beraud, Kersti Tepp, Sophie Pelloux, Siham Chahboun, et al.. Comparative analysis of the bioenergetics of adult cardiomyocytes and nonbeating HL-1 cells: respiratory chain activities, glycolytic enzyme profiles, and metabolic fluxes.. Canadian Journal of Physiology and Pharmacology, NRC Research Press, 2009, 87 (4), pp.318-26. 〈10.1139/y09-018〉. 〈inserm-00391388〉

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